Effects of nicotine and cotinine on expression of adhesion molecules in bone marrow–derived cells
| Cell population . | FSC/SSC . | Treatment . | Control . | CD44 . | VCAM . | α4 . | β2 . | β7 . |
|---|---|---|---|---|---|---|---|---|
| BMCs | 323 ± 53/185 ± 2 | NT | 4 ± 2.6/6.8 ± 1.8 | 73 ± 11/46 ± 6.5 | 8.5 ± 6.8/7.4 ± 0.3 | 38 ± 0.5/16 ± 0.7 | 5.7 ± 5.3/6.6 ± 1 | 8.4 ± 4.6/7 ± 1.4 |
| NC | 4.5 ± 2.8/6.9 ± 1.8 | 75 ± 14/52 ± 1.4 | 14.6 ± 4/9.2 ± 1.7 | 48 ± 5/23 ± 2.6 | 13 ± 6/9 ± 1.8 | 11 ± 9/7.7 ± 0.5 | ||
| CN | 5 ± 1.3/6.7 ± 1.6 | 74 ± 2/49 ± 20 | 13 ± 4.5/8 ± 1 | 39 ± 12/15 ± 5 | 6.5 ± 1.8/6.5 ± 1 | 10 ± 2.4/5 ± 3 | ||
| 528 ± 76/204 ± 13 | NT | 7.3 ± 2/59 ± 8 | 97.7 ± 2.8/480 ± 83 | 18.3 ± 5/82 ± 24 | 94.8 ± 2.4/330 ± 16 | 17 ± 9/86 ± 9 | 9.4 ± 3.6/56 ± 15 | |
| NC | 2 ± 1.4/49 ± 10.5 | 99 ± 0.06/448 ± 29 | 16.3 ± 1.4/72 ± 1.3 | 96 ± 2/298 ± 17 | 16.6 ± 5.8/67 ± 18 | 8.7 ± 1.6/45 ± 10 | ||
| CN | 3.5 ± 2/47.6 ± 3.4 | 98 ± 1.6/470 ± 44 | 20 ± 2.5/78 ± 3.3 | 94.7 ± 2/308 ± 26 | 15 ± 2.3/83 ± 2 | 7.2 ± 5/45 ± 16 | ||
| 836 ± 69/624 ± 4 | NT | 4.2 ± 1/82 ± 13 | 99 ± 1.2/2534 ± 116 | 22 ± 8.4/119 ± 45 | 41 ± 8.6/232 ± 10 | 57.5 ± 15/263 ± 35 | 6 ± 2/49 ± 9 | |
| NC | 7.7 ± 0.05/78 ± 11 | 99 ± 0.3/2148 ± 75 | 39 ± 13/109 ± 33 | 68 ± 11/250 ± 2.5 | 85 ± 2/293 ± 40 | 8.7 ± 1/38.2 ± 5.7 | ||
| CN | 6.3 ± 0.6/78 ± 6 | 99 ± 0.04/2677 ± 96 | 34 ± 9/110 ± 20 | 57.3 ± 1/240 ± 18 | 81 ± 7/260 ± 28 | 5.9 ± 3/42 ± 4.7 | ||
| 328 ± 23/572 ± 60 | NT | 5 ± 4/5.6 ± 1.5 | 78.3 ± 13/122 ± 1.2 | 9.5 ± 9/6.2 ± 0.6 | 27.9 ± 14/10 ± 0.6 | 6.2 ± 7/5.4 ± 1 | 7.5 ± 5.6/6 ± 1.6 | |
| NC | 5.3 ± 4.8/5.3 ± 1.5 | 77.4 ± 11/120 ± 8 | 9.9 ± 6.9/5.7 ± 0.7 | 27.5 ± 12/9.6 ± 0.4 | 10 ± 7/6 ± 1 | 8.6 ± 5.5/5.6 ± 1.2 | ||
| CN | 5.5 ± 0.7/5.2 ± 1.3 | 79 ± 2.8/117 ± 16 | 10 ± 6.8/5.8 ± 1 | 28 ± 8/10 ± 0.4 | 6 ± 3/5.7 ± 1 | 8 ± 2/6 ± 0.7 | ||
| LTBMCs | 625 ± 110/368 ± 150 | NT | 3 ± 1.2/4 ± 0.1 | 99 ± 0.9/203 ± 10 | 20.4 ± 3/7.2 ± 0.5 | 82 ± 15/26 ± 8 | 90.2 ± 5/31.5 ± 7.6 | 10 ± 2.8/5 ± 0.4 |
| NC | 3 ± 2/3.7 ± 0.4 | 97 ± 3.2/159 ± 8 | 19.3 ± 1.2/6.7 ± 0.5 | 84.5 ± 14/26 ± 7 | 90.5 ± 6/30.5 ± 5.29 | 9 ± 0.1/4.8 ± 0.04 | ||
| CN | 2.6 ± 2.2/3.9 ± 0.2 | 99 ± 0.6/189 ± 12 | 20.3 ± 1.5/7 ± 0.5 | 81 ± 14.8/24.6 ± 6 | 92.6 ± 0.7/32 ± 0.3 | 7.6 ± 1.5/4.7 ± 0.8 | ||
| 92 ± 10/124 ± 53 | NT | 2.3 ± 1.5/8.3 ± 2.6 | 24.4 ± 6.8/24 ± 6 | 27 ± 2.8/23 ± 6.3 | 17 ± 3.5/15 ± 3.7 | 18 ± 3/16 ± 4.4 | 7.7 ± 4/11 ± 2.3 | |
| NC | 1.6 ± 0.4/8.7 ± 3.8 | 24 ± 3.8/23 ± 6.7 | 23 ± 0.4/20 ± 7.2 | 16.8 ± 0.4/16 ± 5.4 | 17.6 ± 0.5/16.5 ± 6 | 7 ± 2.7/12 ± 3.8 | ||
| CN | 3 ± 1.6/9.5 ± 4 | 23.3 ± 4/22 ± 6.9 | 26.4 ± 6.3/21 ± 5.6 | 14.5 ± 0.4/14 ± 5.7 | 19 ± 4.7/17 ± 2.9 | 7.3 ± 3/11 ± 3.8 | ||
| PBMDMs | 495 ± 67/561 ± 26 | NT | 3.2 ± 0.04/7 ± 1.4 | 83 ± 2/27.9 ± 5.6 | 8 ± 1.2/9.4 ± 1.2 | 30.4 ± 5.2/14 ± 1.4 | 82 ± 3.6/25 ± 2.6 | 3.6 ± 0.4/7.6 ± 1.7 |
| NC | 2 ± 0.4/5.8 ± 2 | 83.7 ± 18/24 ± 8 | 7.7 ± 3.3/7.5 ± 2 | 27.6 ± 9/11 ± 2.5 | 71 ± 14/19 ± 6 | 3.2 ± 0.6/6.3 ± 1.6 | ||
| CN | 3.3 ± 0.3/6 ± 1.2 | 84.5 ± 0.9/27 ± 2.5 | 8.3 ± 0.1/9 ± 1.2 | 23 ± 12/12 ± 6 | 83 ± 10/26 ± 11 | 7 ± 5/8.2 ± 2.8 | ||
| PBMDFs | 188 ± 59/480 ± 120 | NT | 2.5 ± 0.7/2.6 ± 7.3 | 66 ± 4/355 ± 58 | 16 ± 0.6/53 ± 13 | 5 ± 1.3/32 ± 10 | 6.5 ± 1.4/30 ± 13 | 7 ± 2.4/36 ± 9 |
| NC | 2.7 ± 1.7/21 ± 7 | 48 ± 32.4/156 ± 22 | 12 ± 0.2/44 ± 9 | 6 ± 2/27 ± 13 | 5 ± 0.2/25 ± 11 | 4.3 ± 0.3/33 ± 2.8 | ||
| CN | 2.6 ± 0.8/24 ± 6.4 | 59 ± 5.4/296 ± 13 | 13 ± 2/52 ± 0.1 | 6.8 ± 0.05/30 ± 7 | 6 ± 0.3/28 ± 0.6 | 6 ± 0.9/33 ± 5 | ||
| MS-5 | 460 ± 61/358 ± 44 | NT | 3 ± 1.8/4 ± 0.5 | 81 ± 10/76.9 ± 28 | 3.5 ± 1.2/3.9 ± 0.4 | 5 ± 3.4/4 ± 0.6 | 1.4 ± 1.4/3.5 ± 0.3 | 7 ± 4.8/4 ± 0.7 |
| NC | 2.7 ± 1.4/4 ± 0.2 | 59.6 ± 3/22 ± 12 | 7 ± 5.4/4.7 ± 0.9 | 10 ± 11/5 ± 1.4 | 2 ± 1.3/4 ± 0.4 | 17.4 ± 5.4/14 ± 0.5 | ||
| CN | 3.2 ± 1.2/3.8 ± 0.6 | 71 ± 17/77 ± 9 | 7.5 ± 4.8/4.6 ± 0.5 | 3.4 ± 2.5/4.2 ± 0.9 | 2.5 ± 0.8/3.6 ± 0.6 | 19 ± 4.2/10 ± 7 |
| Cell population . | FSC/SSC . | Treatment . | Control . | CD44 . | VCAM . | α4 . | β2 . | β7 . |
|---|---|---|---|---|---|---|---|---|
| BMCs | 323 ± 53/185 ± 2 | NT | 4 ± 2.6/6.8 ± 1.8 | 73 ± 11/46 ± 6.5 | 8.5 ± 6.8/7.4 ± 0.3 | 38 ± 0.5/16 ± 0.7 | 5.7 ± 5.3/6.6 ± 1 | 8.4 ± 4.6/7 ± 1.4 |
| NC | 4.5 ± 2.8/6.9 ± 1.8 | 75 ± 14/52 ± 1.4 | 14.6 ± 4/9.2 ± 1.7 | 48 ± 5/23 ± 2.6 | 13 ± 6/9 ± 1.8 | 11 ± 9/7.7 ± 0.5 | ||
| CN | 5 ± 1.3/6.7 ± 1.6 | 74 ± 2/49 ± 20 | 13 ± 4.5/8 ± 1 | 39 ± 12/15 ± 5 | 6.5 ± 1.8/6.5 ± 1 | 10 ± 2.4/5 ± 3 | ||
| 528 ± 76/204 ± 13 | NT | 7.3 ± 2/59 ± 8 | 97.7 ± 2.8/480 ± 83 | 18.3 ± 5/82 ± 24 | 94.8 ± 2.4/330 ± 16 | 17 ± 9/86 ± 9 | 9.4 ± 3.6/56 ± 15 | |
| NC | 2 ± 1.4/49 ± 10.5 | 99 ± 0.06/448 ± 29 | 16.3 ± 1.4/72 ± 1.3 | 96 ± 2/298 ± 17 | 16.6 ± 5.8/67 ± 18 | 8.7 ± 1.6/45 ± 10 | ||
| CN | 3.5 ± 2/47.6 ± 3.4 | 98 ± 1.6/470 ± 44 | 20 ± 2.5/78 ± 3.3 | 94.7 ± 2/308 ± 26 | 15 ± 2.3/83 ± 2 | 7.2 ± 5/45 ± 16 | ||
| 836 ± 69/624 ± 4 | NT | 4.2 ± 1/82 ± 13 | 99 ± 1.2/2534 ± 116 | 22 ± 8.4/119 ± 45 | 41 ± 8.6/232 ± 10 | 57.5 ± 15/263 ± 35 | 6 ± 2/49 ± 9 | |
| NC | 7.7 ± 0.05/78 ± 11 | 99 ± 0.3/2148 ± 75 | 39 ± 13/109 ± 33 | 68 ± 11/250 ± 2.5 | 85 ± 2/293 ± 40 | 8.7 ± 1/38.2 ± 5.7 | ||
| CN | 6.3 ± 0.6/78 ± 6 | 99 ± 0.04/2677 ± 96 | 34 ± 9/110 ± 20 | 57.3 ± 1/240 ± 18 | 81 ± 7/260 ± 28 | 5.9 ± 3/42 ± 4.7 | ||
| 328 ± 23/572 ± 60 | NT | 5 ± 4/5.6 ± 1.5 | 78.3 ± 13/122 ± 1.2 | 9.5 ± 9/6.2 ± 0.6 | 27.9 ± 14/10 ± 0.6 | 6.2 ± 7/5.4 ± 1 | 7.5 ± 5.6/6 ± 1.6 | |
| NC | 5.3 ± 4.8/5.3 ± 1.5 | 77.4 ± 11/120 ± 8 | 9.9 ± 6.9/5.7 ± 0.7 | 27.5 ± 12/9.6 ± 0.4 | 10 ± 7/6 ± 1 | 8.6 ± 5.5/5.6 ± 1.2 | ||
| CN | 5.5 ± 0.7/5.2 ± 1.3 | 79 ± 2.8/117 ± 16 | 10 ± 6.8/5.8 ± 1 | 28 ± 8/10 ± 0.4 | 6 ± 3/5.7 ± 1 | 8 ± 2/6 ± 0.7 | ||
| LTBMCs | 625 ± 110/368 ± 150 | NT | 3 ± 1.2/4 ± 0.1 | 99 ± 0.9/203 ± 10 | 20.4 ± 3/7.2 ± 0.5 | 82 ± 15/26 ± 8 | 90.2 ± 5/31.5 ± 7.6 | 10 ± 2.8/5 ± 0.4 |
| NC | 3 ± 2/3.7 ± 0.4 | 97 ± 3.2/159 ± 8 | 19.3 ± 1.2/6.7 ± 0.5 | 84.5 ± 14/26 ± 7 | 90.5 ± 6/30.5 ± 5.29 | 9 ± 0.1/4.8 ± 0.04 | ||
| CN | 2.6 ± 2.2/3.9 ± 0.2 | 99 ± 0.6/189 ± 12 | 20.3 ± 1.5/7 ± 0.5 | 81 ± 14.8/24.6 ± 6 | 92.6 ± 0.7/32 ± 0.3 | 7.6 ± 1.5/4.7 ± 0.8 | ||
| 92 ± 10/124 ± 53 | NT | 2.3 ± 1.5/8.3 ± 2.6 | 24.4 ± 6.8/24 ± 6 | 27 ± 2.8/23 ± 6.3 | 17 ± 3.5/15 ± 3.7 | 18 ± 3/16 ± 4.4 | 7.7 ± 4/11 ± 2.3 | |
| NC | 1.6 ± 0.4/8.7 ± 3.8 | 24 ± 3.8/23 ± 6.7 | 23 ± 0.4/20 ± 7.2 | 16.8 ± 0.4/16 ± 5.4 | 17.6 ± 0.5/16.5 ± 6 | 7 ± 2.7/12 ± 3.8 | ||
| CN | 3 ± 1.6/9.5 ± 4 | 23.3 ± 4/22 ± 6.9 | 26.4 ± 6.3/21 ± 5.6 | 14.5 ± 0.4/14 ± 5.7 | 19 ± 4.7/17 ± 2.9 | 7.3 ± 3/11 ± 3.8 | ||
| PBMDMs | 495 ± 67/561 ± 26 | NT | 3.2 ± 0.04/7 ± 1.4 | 83 ± 2/27.9 ± 5.6 | 8 ± 1.2/9.4 ± 1.2 | 30.4 ± 5.2/14 ± 1.4 | 82 ± 3.6/25 ± 2.6 | 3.6 ± 0.4/7.6 ± 1.7 |
| NC | 2 ± 0.4/5.8 ± 2 | 83.7 ± 18/24 ± 8 | 7.7 ± 3.3/7.5 ± 2 | 27.6 ± 9/11 ± 2.5 | 71 ± 14/19 ± 6 | 3.2 ± 0.6/6.3 ± 1.6 | ||
| CN | 3.3 ± 0.3/6 ± 1.2 | 84.5 ± 0.9/27 ± 2.5 | 8.3 ± 0.1/9 ± 1.2 | 23 ± 12/12 ± 6 | 83 ± 10/26 ± 11 | 7 ± 5/8.2 ± 2.8 | ||
| PBMDFs | 188 ± 59/480 ± 120 | NT | 2.5 ± 0.7/2.6 ± 7.3 | 66 ± 4/355 ± 58 | 16 ± 0.6/53 ± 13 | 5 ± 1.3/32 ± 10 | 6.5 ± 1.4/30 ± 13 | 7 ± 2.4/36 ± 9 |
| NC | 2.7 ± 1.7/21 ± 7 | 48 ± 32.4/156 ± 22 | 12 ± 0.2/44 ± 9 | 6 ± 2/27 ± 13 | 5 ± 0.2/25 ± 11 | 4.3 ± 0.3/33 ± 2.8 | ||
| CN | 2.6 ± 0.8/24 ± 6.4 | 59 ± 5.4/296 ± 13 | 13 ± 2/52 ± 0.1 | 6.8 ± 0.05/30 ± 7 | 6 ± 0.3/28 ± 0.6 | 6 ± 0.9/33 ± 5 | ||
| MS-5 | 460 ± 61/358 ± 44 | NT | 3 ± 1.8/4 ± 0.5 | 81 ± 10/76.9 ± 28 | 3.5 ± 1.2/3.9 ± 0.4 | 5 ± 3.4/4 ± 0.6 | 1.4 ± 1.4/3.5 ± 0.3 | 7 ± 4.8/4 ± 0.7 |
| NC | 2.7 ± 1.4/4 ± 0.2 | 59.6 ± 3/22 ± 12 | 7 ± 5.4/4.7 ± 0.9 | 10 ± 11/5 ± 1.4 | 2 ± 1.3/4 ± 0.4 | 17.4 ± 5.4/14 ± 0.5 | ||
| CN | 3.2 ± 1.2/3.8 ± 0.6 | 71 ± 17/77 ± 9 | 7.5 ± 4.8/4.6 ± 0.5 | 3.4 ± 2.5/4.2 ± 0.9 | 2.5 ± 0.8/3.6 ± 0.6 | 19 ± 4.2/10 ± 7 |
Cells were not treated or were treated with 10−4 M (for 2 hours) nicotine or cotinine. Expression of adhesion molecules was evaluated by FACS and expressed as means ± SD of the percentage of the gated cell population/mean fluorescence intensity. Data from 2 to 5 independent experiments are presented.
FSC indicates forward light scatter; SSC, side light scatter; VCAM, vascular cell adhesion molecule; BMCs, bone marrow cells; NT, no treatment; NC, nicotine; CN, cotinine; LTBMCs, long-term bone marrow cultures; PBMDMs, primary bone marrow–derived macrophages; PBMDFs, primary bone marrow–derived fibroblasts.