A comparison of the adhesion of nonhemopoietic cell lines to intercellular adhesion molecule-4Fc with their integrin profiles determined by flow cytometry
| Cell line (activation) . | Adhesion to Fc fusion proteins, percentage of input cells . | Integrin expression, MFI . | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ICAM4Fc . | NCAMFc . | β1 . | β2 . | β3 . | β4 . | α1 . | α2 . | α3 . | α4 . | α5 . | α6 . | αV . | αVβ3 . | αVβ5 . | C . | |
| FLY (+PMA) | 71 | 5 | 743 | 7 | 21 | 7 | 15 | 434 | 274 | 6 | 296 | 73 | 116 | 49 | 93 | 2 |
| HFFF (+PMA) | 51 | 7 | 307 | 3 | 18 | 4 | 94 | 74 | 65 | 76 | 142 | 9 | 90 | 27 | 33 | 4 |
| ECV304 (−PMA) | 28 | 3 | 754 | 5 | 47 | 170 | 5 | 147 | 163 | 9 | 43 | 30 | 155 | 55 | 136 | 4 |
| SK-HEP1 (+PMA) | 48 | 3 | 1098 | 3 | 4 | 75 | 9 | 243 | 353 | 126 | 90 | 70 | 50 | 5 | 71 | 4 |
| HUVEC (−PMA) | 18 | 6 | 389 | NT | NT | NT | 13 | 163 | 69 | 5 | 170 | 5 | NT | 165 | 33 | 4 |
| DX3 (+PMA) | 65 | 7 | 347 | 3 | 17 | 5 | 33 | 107 | 105 | 68 | 37 | 10 | 36 | 25 | 26 | 3 |
| HT29 (−PMA) | 39 | 3 | 332 | 3 | 3 | 311 | 117 | 158 | 150 | 3 | 3 | 76 | 109 | 3 | 134 | 3 |
| 293 (+PMA) | 51 | 20 | 413 | 3 | 5 | 11 | 146 | 98 | 74 | 28 | 82 | 6 | 60 | 7 | 12 | 3 |
| COS (+PMA) | 80 | 9 | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT |
| CHO (−PMA) | 33 | 3 | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT |
| Cell line (activation) . | Adhesion to Fc fusion proteins, percentage of input cells . | Integrin expression, MFI . | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ICAM4Fc . | NCAMFc . | β1 . | β2 . | β3 . | β4 . | α1 . | α2 . | α3 . | α4 . | α5 . | α6 . | αV . | αVβ3 . | αVβ5 . | C . | |
| FLY (+PMA) | 71 | 5 | 743 | 7 | 21 | 7 | 15 | 434 | 274 | 6 | 296 | 73 | 116 | 49 | 93 | 2 |
| HFFF (+PMA) | 51 | 7 | 307 | 3 | 18 | 4 | 94 | 74 | 65 | 76 | 142 | 9 | 90 | 27 | 33 | 4 |
| ECV304 (−PMA) | 28 | 3 | 754 | 5 | 47 | 170 | 5 | 147 | 163 | 9 | 43 | 30 | 155 | 55 | 136 | 4 |
| SK-HEP1 (+PMA) | 48 | 3 | 1098 | 3 | 4 | 75 | 9 | 243 | 353 | 126 | 90 | 70 | 50 | 5 | 71 | 4 |
| HUVEC (−PMA) | 18 | 6 | 389 | NT | NT | NT | 13 | 163 | 69 | 5 | 170 | 5 | NT | 165 | 33 | 4 |
| DX3 (+PMA) | 65 | 7 | 347 | 3 | 17 | 5 | 33 | 107 | 105 | 68 | 37 | 10 | 36 | 25 | 26 | 3 |
| HT29 (−PMA) | 39 | 3 | 332 | 3 | 3 | 311 | 117 | 158 | 150 | 3 | 3 | 76 | 109 | 3 | 134 | 3 |
| 293 (+PMA) | 51 | 20 | 413 | 3 | 5 | 11 | 146 | 98 | 74 | 28 | 82 | 6 | 60 | 7 | 12 | 3 |
| COS (+PMA) | 80 | 9 | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT |
| CHO (−PMA) | 33 | 3 | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT | NT |
The monkey (COS) and hamster (CHO) cell lines were not tested (NT) with antibodies against human integrins.
MFI indicates mean fluorescence intensity.