Adhesion of hemopoietic cells to Fc fusion proteins
| Cell line (activation) . | Adhesion to Fc fusion proteins, percentage of input cells . | Expression of integrin subunits, MFI . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ICAM1Fc . | ICAM2Fc . | ICAM3Fc . | ICAM4Fc . | IC4R52EFc . | IC4S50GFc . | IC4T91QFc . | NCAMFc . | αL . | β2 . | α4 . | β1 . | αV . | C . | |
| HEL (−PMA) | 20 | 16 | 10 | 62 | 64 | 59 | 60 | 11 | 14 | 8 | 283 | 308 | 43 | 4 |
| K562 (+PMA) | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 8 | 4 | 3 | 112 | 9 | 3 |
| KG1a (+PMA) | 89 | 78 | 73 | 34 | 27 | 27 | 30 | 9 | 285 | 252 | 379 | 280 | 4 | 2 |
| HL-60 (+PMA) | 21 | 8 | 5 | 5 | 5 | 5 | 4 | 3 | 31 | 26 | 158 | 248 | 4 | 4 |
| THP1 (+PMA) | 96 | 64 | 38 | 53 | 50 | 40 | 43 | 23 | 151 | 141 | 95 | 155 | 5 | 3 |
| U937 (+PMA) | 51 | 12 | 4 | 5 | 3 | 5 | 4 | 4 | 28 | 22 | 365 | 385 | 3 | 2 |
| Jurkat (+PMA) | 67 | 34 | 24 | 26 | 21 | 23 | 22 | 4 | 60 | 51 | 363 | 558 | 15 | 3 |
| Molt-4 (+PMA) | 5 | 5 | NT | 29 | 32 | 18 | 19 | 4 | 34 | 27 | 580 | 452 | 5 | 2 |
| EBV-LCL (+PMA) | 42 | 27 | 19 | 10 | 10 | 9 | 9 | 10 | 94 | 75 | 47 | 172 | 13 | 3 |
| IM9 (+PMA) | 61 | 19 | 9 | 9 | 9 | 8 | 9 | 7 | 77 | 56 | 288 | 144 | 15 | 3 |
| Daudi (−PMA) | 24 | 14 | NT | 9 | 9 | 10 | 10 | 6 | 31 | 21 | 107 | 129 | 2 | 2 |
| Raji (−PMA) | 28 | 7 | NT | 10 | 14 | 9 | 13 | 4 | 15 | 11 | 172 | 120 | 3 | 3 |
| Cell line (activation) . | Adhesion to Fc fusion proteins, percentage of input cells . | Expression of integrin subunits, MFI . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ICAM1Fc . | ICAM2Fc . | ICAM3Fc . | ICAM4Fc . | IC4R52EFc . | IC4S50GFc . | IC4T91QFc . | NCAMFc . | αL . | β2 . | α4 . | β1 . | αV . | C . | |
| HEL (−PMA) | 20 | 16 | 10 | 62 | 64 | 59 | 60 | 11 | 14 | 8 | 283 | 308 | 43 | 4 |
| K562 (+PMA) | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 8 | 4 | 3 | 112 | 9 | 3 |
| KG1a (+PMA) | 89 | 78 | 73 | 34 | 27 | 27 | 30 | 9 | 285 | 252 | 379 | 280 | 4 | 2 |
| HL-60 (+PMA) | 21 | 8 | 5 | 5 | 5 | 5 | 4 | 3 | 31 | 26 | 158 | 248 | 4 | 4 |
| THP1 (+PMA) | 96 | 64 | 38 | 53 | 50 | 40 | 43 | 23 | 151 | 141 | 95 | 155 | 5 | 3 |
| U937 (+PMA) | 51 | 12 | 4 | 5 | 3 | 5 | 4 | 4 | 28 | 22 | 365 | 385 | 3 | 2 |
| Jurkat (+PMA) | 67 | 34 | 24 | 26 | 21 | 23 | 22 | 4 | 60 | 51 | 363 | 558 | 15 | 3 |
| Molt-4 (+PMA) | 5 | 5 | NT | 29 | 32 | 18 | 19 | 4 | 34 | 27 | 580 | 452 | 5 | 2 |
| EBV-LCL (+PMA) | 42 | 27 | 19 | 10 | 10 | 9 | 9 | 10 | 94 | 75 | 47 | 172 | 13 | 3 |
| IM9 (+PMA) | 61 | 19 | 9 | 9 | 9 | 8 | 9 | 7 | 77 | 56 | 288 | 144 | 15 | 3 |
| Daudi (−PMA) | 24 | 14 | NT | 9 | 9 | 10 | 10 | 6 | 31 | 21 | 107 | 129 | 2 | 2 |
| Raji (−PMA) | 28 | 7 | NT | 10 | 14 | 9 | 13 | 4 | 15 | 11 | 172 | 120 | 3 | 3 |
Expression of integrin subunits αL, β2, α4, β1, and αV determined by flow cytometry.
ICAM indicates intercellular adhesion molecule; NCAMFc, neural cell adhesion molecule control protein; PMA, phorbol myristate acetate; MFI, mean fluorescence intensity measured by flow cytometry; NT, not tested; C, control antibody.