Cobblestone-area—forming cells induced by BMP-4 and hematopoietic cytokines
| Differentiation treatment . | Secondary CAFCs/100 000 cells . | ||
|---|---|---|---|
| Day 114-150 . | Day 224-150 . | Day 294-150 . | |
| BMP-4, SCF, IL-3, IL-6, VEGF, G-CSF, and Epo | 3 | 2 | — |
| BMP-4, SCF, IL-3, IL-6, VEGF, G-CSF, Epo, and thrombopoietin | 4 | 2 | 0 |
| BMP-4, SCF, IL-3, IL-6, VEGF, G-CSF, Epo, and Flt3 ligand | 34 | 102 | 158 |
| Differentiation treatment . | Secondary CAFCs/100 000 cells . | ||
|---|---|---|---|
| Day 114-150 . | Day 224-150 . | Day 294-150 . | |
| BMP-4, SCF, IL-3, IL-6, VEGF, G-CSF, and Epo | 3 | 2 | — |
| BMP-4, SCF, IL-3, IL-6, VEGF, G-CSF, Epo, and thrombopoietin | 4 | 2 | 0 |
| BMP-4, SCF, IL-3, IL-6, VEGF, G-CSF, Epo, and Flt3 ligand | 34 | 102 | 158 |
Nonadherent hematopoietic cells from primary ES cell differentiation cultures were replated on confluent S17 stromal cells in the presence of hematopoietic growth factors (SCF, IL-3, granulocyte-macrophage colony-stimulating factor (CM-CSF), IL-6, G-CSF, VEGF, Flt3 ligand, and Epo). The secondary cobblestone colonies were counted after 12 to 14 days of culture.
Days after expansion of hematopoietic clusters in primary culture.