Development of hematopoietic clusters from differentiated rhesus monkey ES cells
| Experiment no. . | Hematopoietic clusters* . | Expandable hematopoietic clusters† . | ||
|---|---|---|---|---|
| Control . | BMP-4 . | Control . | BMP-4 . | |
| 1 | 7 | 32 | 0 | 68 |
| 2 | 0 | 27 | 0 | 65 |
| 3 | 0 | 7 | 0 | 43 |
| 4 | 2 | 32 | 2 | 57 |
| 5 | 10 | 92 | 18 | 71 |
| 6 | 0 | 52 | 6 | 142 |
| 7 | 0 | 6 | 0 | 18 |
| 8 | 0 | 3 | 0 | 15 |
| 9 | 12 | 84 | NT | NT |
| 10 | 1 | 82 | NT | NT |
| 11 | 7 | 62 | NT | NT |
| 12 | 1 | 106 | NT | NT |
| 13 | 1 | 47 | NT | NT |
| Experiment no. . | Hematopoietic clusters* . | Expandable hematopoietic clusters† . | ||
|---|---|---|---|---|
| Control . | BMP-4 . | Control . | BMP-4 . | |
| 1 | 7 | 32 | 0 | 68 |
| 2 | 0 | 27 | 0 | 65 |
| 3 | 0 | 7 | 0 | 43 |
| 4 | 2 | 32 | 2 | 57 |
| 5 | 10 | 92 | 18 | 71 |
| 6 | 0 | 52 | 6 | 142 |
| 7 | 0 | 6 | 0 | 18 |
| 8 | 0 | 3 | 0 | 15 |
| 9 | 12 | 84 | NT | NT |
| 10 | 1 | 82 | NT | NT |
| 11 | 7 | 62 | NT | NT |
| 12 | 1 | 106 | NT | NT |
| 13 | 1 | 47 | NT | NT |
In control cultures, rhesus monkey ES cells were cocultured with S17 stromal cells in the presence of hematopoietic cytokines. BMP-4 was added at a concentration of 20 ng/mL. For experiments 1 to 4, SCF, IL-3, IL-6, G-CSF, VEGF, and Epo were included. For experiments 5 to 13, Flt3 ligand was also added.
NT indicates not tested.
Values are the numbers of hematopoietic clusters/4000 ES cells on day 13 of primary culture.
Values are the numbers of proliferated clusters after the appearance of primary hematopoietic clusters on day 13 of differentiation culture; expansion growth was continued for an additional 5 to 7 days.