Dose-response analysis of hematopoietic differentiation induced by bone morphogenetic protein 4
| Experiment no. . | BMP-4 dose (ng/mL) . | |||
|---|---|---|---|---|
| 0 . | 10 . | 20 . | 40 . | |
| 1 | 7 | 21 | 32 | NT |
| 2 | 2 | 14 | 32 | NT |
| 3 | 0 | 31 | 52 | 11 |
| Experiment no. . | BMP-4 dose (ng/mL) . | |||
|---|---|---|---|---|
| 0 . | 10 . | 20 . | 40 . | |
| 1 | 7 | 21 | 32 | NT |
| 2 | 2 | 14 | 32 | NT |
| 3 | 0 | 31 | 52 | 11 |
Rhesus monkey embryonic stem (ES) cells were cocultured with S17 stromal cells containing stem cell factor (SCF), interleukin 3 (IL-3), interleukin 6 (IL-6), vascular endothelial growth factor (VEGF), granulocyte colony-stimulating factor (G-CSF), and erythropoietin (Epo). In experiment 3, Flt3 ligand was included with the hematopoietic cytokines. Values are the numbers of hematopoietic-like clusters/4000 rhesus monkey ES cells on day 13 of culture.
NT indicates not tested.