Donor CD4+ cell numbers in allogeneic major histocompatibility complex-mismatched recipients early following bone marrow transplantation of B6 wild-type versus B6 cytotoxic-deficient T cells
| Donor* . | Recipient (8.5 Gy) . | Day 5 . | Day 7 . | ||
|---|---|---|---|---|---|
| Spleen (× 106) . | MLN (× 106) . | Spleen (× 106) . | MLN (× 106) . | ||
| B6 CD4 (group A) | BALB/c | 0.28 ± 0.03† | 1.9 ± 0.02 | 1.41 ± 0.15 | 0.17 ± 0.025 |
| B6-cdd CD4 (group B) | BALB/c | 0.01 ± 0.003‡ | 0.04 ± 0.02‡ | 0.44 ± 0.024‡ | 0.05 ± 0.01‡ |
| Donor* . | Recipient (8.5 Gy) . | Day 5 . | Day 7 . | ||
|---|---|---|---|---|---|
| Spleen (× 106) . | MLN (× 106) . | Spleen (× 106) . | MLN (× 106) . | ||
| B6 CD4 (group A) | BALB/c | 0.28 ± 0.03† | 1.9 ± 0.02 | 1.41 ± 0.15 | 0.17 ± 0.025 |
| B6-cdd CD4 (group B) | BALB/c | 0.01 ± 0.003‡ | 0.04 ± 0.02‡ | 0.44 ± 0.024‡ | 0.05 ± 0.01‡ |
MLN indicates mesenteric lymph nodes.
1 × 106 CD4+ T cells were prepared by means of the Miltenyi bead selection system described in “Materials and methods.” The percentage of CD4+ T cells was as follows: B6 = 96.5%; B6-cdd = 95.2%. CD4+ T cells were infused together with 5 × 106 B6-Ly5.1 TCD-bone marrow cells. Two mice per group were pooled, and the average cell number is presented. Control syngeneic bone marrow transplantations were performed with B6-Ly5.1 CD4+ T cells → syngeneic B6 recipients. The cell numbers in the spleen were 0.56 × 106and 2.7 × 106 at days 5 and 7, respectively.
T-cell numbers were determined by obtaining the percentage of CD4+ donor cells via 3-color flow cytometric analysis following staining with anti-H-2b, anti-Ly5.2 (donor), and anti-CD4 monoclonal antibodies and multiplying the percentage of H-2b+, Ly5.2+, and CD4+ cells by the total number of nucleated cells in each tissue.
P < .05.