Onset time (days) of CD4+ T-cell-induced graft-versus-host-disease-associated clinical changes
| Donor . | CD4+ Cell No.* . | Onset time† in days by type of clinical changes . | |||
|---|---|---|---|---|---|
| Diarrhea . | Haunched . | Alopecia . | Ruffled fur . | ||
| B6 | 2.5 × 106 | 11.8 ± 1.0 | 12.4 ± 0.4 | 13.2 ± 0.7 | 5.6 ± 0.2 |
| B6-cdd | 2.5 × 106 | 11.4 ± 1.0 | 12.8 ± 0.5 | 12.2 ± 0.2 | 6.2 ± 0.5 |
| P‡ | .780 | .545 | .226 | .305 | |
| B6 | 0.25 × 106 | 15.3 ± 3.7 | 14.3 ± 2.7 | 19.0 ± 2.0 | 10.6 ± 0.6 |
| B6-cdd | 0.25 × 106 | 22.8 ± 2.6 | 27.6 ± 1.98 | 25.2 ± 1.4 | 18.2 ± 1.02 |
| P‡ | .140 | < .05 | < .05 | < .05 | |
| Donor . | CD4+ Cell No.* . | Onset time† in days by type of clinical changes . | |||
|---|---|---|---|---|---|
| Diarrhea . | Haunched . | Alopecia . | Ruffled fur . | ||
| B6 | 2.5 × 106 | 11.8 ± 1.0 | 12.4 ± 0.4 | 13.2 ± 0.7 | 5.6 ± 0.2 |
| B6-cdd | 2.5 × 106 | 11.4 ± 1.0 | 12.8 ± 0.5 | 12.2 ± 0.2 | 6.2 ± 0.5 |
| P‡ | .780 | .545 | .226 | .305 | |
| B6 | 0.25 × 106 | 15.3 ± 3.7 | 14.3 ± 2.7 | 19.0 ± 2.0 | 10.6 ± 0.6 |
| B6-cdd | 0.25 × 106 | 22.8 ± 2.6 | 27.6 ± 1.98 | 25.2 ± 1.4 | 18.2 ± 1.02 |
| P‡ | .140 | < .05 | < .05 | < .05 | |
CD4+ T cells were prepared by means of the Macs system as described in “Materials and methods.” The percentage of CD4+ T cells was as follows: B6 = 97%; B6-cdd = 98%. The CD4+ T cells were infused together with 5 × 106 B6-Ly5.1 TCD-bone marrow cells into 8.5-Gy BALB/c recipients (n=5).
The onset time was determined as the average time the animals in each group (≥3) exhibited the clinical change (ie, score of 1 or 2).
P < .05 was considered significant.