Intraperitoneal abscess formation following inoculation with Staphylococcus aureus
Group . | N . | NBT (%) . | Intra-abdominal abscess . |
---|---|---|---|
WT | 33 | 100 | 3/33*,† |
X-CGD | 27 | 0 | 12/27* |
Transplantation chimeras | |||
WT/X-CGD | 8 | 5-10 | 5/8† |
10 | 11-20 | 4/10† | |
5 | 21-35 | 0/5 | |
5 | 35-50 | 2/5 | |
MSCV-m91Neo/X-CGD | 4 | 5-10 | 3/4† |
4 | 11-20 | 2/4 | |
WT Transplants | 10 | 100 | 0/10 |
Group . | N . | NBT (%) . | Intra-abdominal abscess . |
---|---|---|---|
WT | 33 | 100 | 3/33*,† |
X-CGD | 27 | 0 | 12/27* |
Transplantation chimeras | |||
WT/X-CGD | 8 | 5-10 | 5/8† |
10 | 11-20 | 4/10† | |
5 | 21-35 | 0/5 | |
5 | 35-50 | 2/5 | |
MSCV-m91Neo/X-CGD | 4 | 5-10 | 3/4† |
4 | 11-20 | 2/4 | |
WT Transplants | 10 | 100 | 0/10 |
X-linked chronic granulomatous disease (X-CGD) mice were transplanted with either mixtures of wild-type (WT) and X-CGD bone marrow (BM) or X-CGD BM transduced with MSCV-m91Neo and mock-transduced X-CGD BM. A separate group of WT mice were also transplanted with WT BM. As described in the “Materials and methods” section, WT, X-CGD, and transplanted mice were inoculated by intraperitoneal injection with 2 × 107Staphylococcus aureus, and abscess formation was scored at 1 week.
P < .005 Fisher exact test (WT vs X-CGD).
P < .05 Fisher exact test (WT vs WT/X-CGD or MSCV-m91Neo/X-CGD).