Aspergillus fumigatus pneumonia in X-CGD transplantation chimera mice
| Group . | Conidia . | N . | NBT (%) . | A fumigatus lung disease . |
|---|---|---|---|---|
| X-CGD | 150-200 | 10 | 0 | 10/10* |
| MSCV-m91Neo/X-CGD | 150-200 | 5 | 1-3 | 5/5 |
| 6 | 5-10 | 4/6†,‡ | ||
| 3 | 11-15 | 0/3*,‡ | ||
| 3 | 18-21 | 0/3*,‡ | ||
| 4 000 | 7 | 19-30 | 0/7 | |
| WT/X-CGD | 150-200 | 7 | 2-3 | 7/71-153 |
| 7 | 4-8 | 0/7†,1-154 | ||
| 5 000 | 6 | 15-33 | 0/6 | |
| 50 000 | 5 | 21-36 | 0/5 |
| Group . | Conidia . | N . | NBT (%) . | A fumigatus lung disease . |
|---|---|---|---|---|
| X-CGD | 150-200 | 10 | 0 | 10/10* |
| MSCV-m91Neo/X-CGD | 150-200 | 5 | 1-3 | 5/5 |
| 6 | 5-10 | 4/6†,‡ | ||
| 3 | 11-15 | 0/3*,‡ | ||
| 3 | 18-21 | 0/3*,‡ | ||
| 4 000 | 7 | 19-30 | 0/7 | |
| WT/X-CGD | 150-200 | 7 | 2-3 | 7/71-153 |
| 7 | 4-8 | 0/7†,1-154 | ||
| 5 000 | 6 | 15-33 | 0/6 | |
| 50 000 | 5 | 21-36 | 0/5 |
X-linked chronic granulomatous disease (X-CGD) mice were transplanted with either mixtures of wild-type (WT) and X-CGD bone marrow (BM) or X-CGD BM transduced with MSCV-m91Neo and mock-transduced X-CGD BM. As described in the “Materials and methods” section, transplanted mice were challenged with the indicated dose of A fumigatus conidia.
P < .001 (X-CGD vs MSCV-m91Neo/X-CGD, 11% to 21% nitroblue tetrazolium [NBT]-positive neutrophils), Fisher exact test.
P = .02 (MSCV-m91Neo/X-CGD, 5% to 10% NBT-positive neutrophils vs WT/X-CGD, 4% to 8% NBT-positive neutrophils), Fisher exact test.
P = .06 (MSCV-m91Neo/X-CGD, 5% to 10% NBT-positive neutrophils vs 11% to 21% NBT-positive neutrophils), Fisher exact test.
Includes data on 4 mice studied previously31and 3 mice in current study.
Includes 6 mice studied previously31 and one in current study.