Fluorescence in situ hybridization of colonies from colony-forming cell and long-term culture-initiating cell assays of sorted cells
| Patient . | CFC . | LTC-IC . | ||||||
|---|---|---|---|---|---|---|---|---|
| SP+CD34+CD38− . | SP−CD34+CD38− . | SP+CD34+CD38− . | SP−CD34+CD38− . | |||||
| No. abnormal/no. analyzed . | Abnormal (%) . | No. abnormal/no. analyzed . | Abnormal (%) . | No. abnormal/no. analyzed . | Abnormal (%) . | No. abnormal/no. analyzed . | Abnormal (%) . | |
| 1 | 0/16 | 0 | 3/7 | 42.9 | 0/17 | 0 | NG | — |
| 2 | 20/20 | 100 | 26/26 | 100 | 14/14 | 100 | 10/10 | 100 |
| 3 | 0/1 | 0 | NG | — | NG | — | NS | — |
| 4 | 0/26 | 0 | ND | — | 0/9 | 0 | ND | — |
| 5 | 0/7 | 0 | 0/5 | 0 | 0/18 | 0 | NG | — |
| 6 | NG | — | NG | — | 0/30 | 0 | 0/20 | 0 |
| 7 | 0/4 | 0 | 3/10 | 30 | 0/17 | 0 | NG | — |
| 8 | 0/9 | 0 | 2/9 | 22.2 | NG | — | NG | — |
| 13 | 1/30 | 3 | NG | — | 0/10 | 0 | NG | — |
| 14 | NG | — | NG | — | NG | — | NS | — |
| Patient . | CFC . | LTC-IC . | ||||||
|---|---|---|---|---|---|---|---|---|
| SP+CD34+CD38− . | SP−CD34+CD38− . | SP+CD34+CD38− . | SP−CD34+CD38− . | |||||
| No. abnormal/no. analyzed . | Abnormal (%) . | No. abnormal/no. analyzed . | Abnormal (%) . | No. abnormal/no. analyzed . | Abnormal (%) . | No. abnormal/no. analyzed . | Abnormal (%) . | |
| 1 | 0/16 | 0 | 3/7 | 42.9 | 0/17 | 0 | NG | — |
| 2 | 20/20 | 100 | 26/26 | 100 | 14/14 | 100 | 10/10 | 100 |
| 3 | 0/1 | 0 | NG | — | NG | — | NS | — |
| 4 | 0/26 | 0 | ND | — | 0/9 | 0 | ND | — |
| 5 | 0/7 | 0 | 0/5 | 0 | 0/18 | 0 | NG | — |
| 6 | NG | — | NG | — | 0/30 | 0 | 0/20 | 0 |
| 7 | 0/4 | 0 | 3/10 | 30 | 0/17 | 0 | NG | — |
| 8 | 0/9 | 0 | 2/9 | 22.2 | NG | — | NG | — |
| 13 | 1/30 | 3 | NG | — | 0/10 | 0 | NG | — |
| 14 | NG | — | NG | — | NG | — | NS | — |
NG, no growth; ND, not done (no sorted cells were placed in the LTC-IC assay); NS, fluorescence in situ hybridization (FISH) analysis on colonies was unsuccessful. See Tables 2 and 4 for other abbreviations.