Table 1. Specific activities of... | American Society of Hematology

Table 1.

Specific activities of recombinant factor XIa in chromogenic substrate assays and factor IX activation assays

	S-2366		Factor IX
	K_m, μM	k_cat, sec⁻¹	K_m, μM	k_cat, min⁻¹	k_cat/K_m
Wild-type	400	200	0.28	10	35.7
Factor XI-R226	350	160	1.47	53.2	36.2
Factor XI-N248	450	180	0.36	8.1	22.5

	S-2366		Factor IX
	K_m, μM	k_cat, sec⁻¹	K_m, μM	k_cat, min⁻¹	k_cat/K_m
Wild-type	400	200	0.28	10	35.7
Factor XI-R226	350	160	1.47	53.2	36.2
Factor XI-N248	450	180	0.36	8.1	22.5

Kinetic parameters for the cleavage of chromogenic substrate S-2366 and the activation of purified factor IX were determined for the activated forms of recombinant wild-type factor XI, factor XI-R226, and factor XI-N248. The assays were conducted as described in “Materials and methods,” and all results are means of duplicate experiments.

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