Comparison of expression level of apparent species-specific genes by semiquantitative reverse transcriptase–polymerase chain reaction
| Specificity (by GFs) . | UniGene cluster ID . | Primer pair . | Hu/Bab intensity ratio (by GFs) . | Hu/Bab intensity ratio (by RT-PCR) . | Gene name . |
|---|---|---|---|---|---|
| Human | Hs.1817 | R05886 | 16.3 | 3.6 | MPO |
| Human | Hs.13818 | R85439 | 6.9 | 1.5 | ESTs |
| Human | Hs.43708 | N25920 | 3.7 | −1.9 | EST |
| Human | Hs.215595 | AA487912 | 3.2 | 5.4 | GNB1 |
| Baboon | Hs.118409 | AA676327 | −21.5 | 1.8 | ESTs |
| Baboon | Hs.107308 | R82595 | −19.3 | 1.2 | cDNA |
| Specificity (by GFs) . | UniGene cluster ID . | Primer pair . | Hu/Bab intensity ratio (by GFs) . | Hu/Bab intensity ratio (by RT-PCR) . | Gene name . |
|---|---|---|---|---|---|
| Human | Hs.1817 | R05886 | 16.3 | 3.6 | MPO |
| Human | Hs.13818 | R85439 | 6.9 | 1.5 | ESTs |
| Human | Hs.43708 | N25920 | 3.7 | −1.9 | EST |
| Human | Hs.215595 | AA487912 | 3.2 | 5.4 | GNB1 |
| Baboon | Hs.118409 | AA676327 | −21.5 | 1.8 | ESTs |
| Baboon | Hs.107308 | R82595 | −19.3 | 1.2 | cDNA |
Negative intensity ratio indicates higher expression in baboon than in human. Primer pairs were named after the GenBank Accession number specifying a cDNA from a specific IMAGE clone spotted on GeneFilter membrane.
GF, GeneFilters; MPO, myeloperoxidase; GNB1, Guanine nucleotide binding protein (G protein), beta polypeptide 1; cDNA, Homo sapiens uncharacterized gene.