Expression of human Ly-9 on lymphoid and myeloid cell lines
. | Positive cells (%) . | Log mean fluorescence intensity . |
---|---|---|
Raji | 76.4 | 31.10 |
Daudi | 99.8 | 36.09 |
CESS | 90.5 | 21.96 |
BEN* | 76.3 | 18.95 |
RPMI 8222 | 21.0 | 7.08 |
JM1 | 13.1 | 4.75 |
Jurkat | 76.0 | 17.37 |
HSB2 | 7.5 | 4.17 |
HL60 | 4.1 | 3.79 |
U937 | 5.4 | 4.20 |
K562 | 12.5 | 4.32 |
. | Positive cells (%) . | Log mean fluorescence intensity . |
---|---|---|
Raji | 76.4 | 31.10 |
Daudi | 99.8 | 36.09 |
CESS | 90.5 | 21.96 |
BEN* | 76.3 | 18.95 |
RPMI 8222 | 21.0 | 7.08 |
JM1 | 13.1 | 4.75 |
Jurkat | 76.0 | 17.37 |
HSB2 | 7.5 | 4.17 |
HL60 | 4.1 | 3.79 |
U937 | 5.4 | 4.20 |
K562 | 12.5 | 4.32 |
Cells were stained with biotinylated HLy-9.1.84 mAb. This was followed by incubation with avidin-phycoerythrin and analysis by flow cytometry. An unreactive mAb was used as negative control. Results represent the percentage of positive cells and the mean of fluorescence intensity. Positive staining appears in boldface.