Table 1.

Expression of human Ly-9 on lymphoid and myeloid cell lines

Positive cells (%)Log mean fluorescence intensity
Raji 76.4 31.10  
Daudi 99.8 36.09 
CESS 90.5 21.96 
BEN* 76.3 18.95  
RPMI 8222 21.0 7.08  
JM1 13.1 4.75 
Jurkat 76.0 17.37  
HSB2 7.5 4.17 
HL60 4.1 3.79  
U937 5.4 4.20 
K562 12.5 4.32 
Positive cells (%)Log mean fluorescence intensity
Raji 76.4 31.10  
Daudi 99.8 36.09 
CESS 90.5 21.96 
BEN* 76.3 18.95  
RPMI 8222 21.0 7.08  
JM1 13.1 4.75 
Jurkat 76.0 17.37  
HSB2 7.5 4.17 
HL60 4.1 3.79  
U937 5.4 4.20 
K562 12.5 4.32 

Cells were stained with biotinylated HLy-9.1.84 mAb. This was followed by incubation with avidin-phycoerythrin and analysis by flow cytometry. An unreactive mAb was used as negative control. Results represent the percentage of positive cells and the mean of fluorescence intensity. Positive staining appears in boldface.

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