MRD Flow provides at least one log greater sensitivity and specificity of detection of CLL cells than conventional flow cytometry and consensus-primer PCR
| Detection of CLL cells . | Conventional flow cytometry . | PCR . | MRD Flow . | |
|---|---|---|---|---|
| 2-Color CD19CD5 . | 4-Color CD19/CD5 κ/λ . | Fluorescent consensus Fr3 primer . | 4-Color CD19/CD5 CD20/CD79b . | |
| Percentage of leukocytes (range) | 1.2 (0.4-2.6) | 0.4 (0.3-0.7) | 0.6 (0.4-0.7) | 0.005 (0.003-0.008) |
| Percentage of total B cells (range) | 35 (33-71) | 33 (12-71) | 52 (33-71) | 0.65 (0.47-1.62) |
| Detection of CLL cells . | Conventional flow cytometry . | PCR . | MRD Flow . | |
|---|---|---|---|---|
| 2-Color CD19CD5 . | 4-Color CD19/CD5 κ/λ . | Fluorescent consensus Fr3 primer . | 4-Color CD19/CD5 CD20/CD79b . | |
| Percentage of leukocytes (range) | 1.2 (0.4-2.6) | 0.4 (0.3-0.7) | 0.6 (0.4-0.7) | 0.005 (0.003-0.008) |
| Percentage of total B cells (range) | 35 (33-71) | 33 (12-71) | 52 (33-71) | 0.65 (0.47-1.62) |
Date shown is the median, maximum, and minimum number of CLL cells as a percentage of total leukocytes (sensitivity) or of total B lymphocytes (specificity) at which each of the assays showed a “positive” result (ie, could identify the presence of CLL cells in the polyclonal background). Criteria for detection of CLL cells are described in the Figure 3 legend.