Hematopoietic tissue parameters
| Parameter . | Csf1op/Csf1op . | +/Csf1op . | Csf1op/Csf1op; TgC/+ . |
|---|---|---|---|
| Bone marrow cellularity, × 10−6cells per femur4-150 | 6.3 ± 0.9 | 19.8 ± 1.5 | 16.2 ± 0.6 |
| Splenic weight, g | 0.083 ± 0.05 | 0.097 ± 0.01 | 0.109 ± 0.03 |
| Splenic cellularity, × 10−9/g4-150 | 2.9 ± 0.6 | 1.5 ± 0.17 | 1.5 ± 0.1 |
| Blood cellularity | |||
| Total leukocytes, × 10−6/mL | 5.9 ± 1.1 | 5.0 ± 0.3 | 4.7 ± 0.6 |
| Monocytes, %4-150 | 0.9 ± 0.2 | 7.3 ± 0.8 | 8.1 ± 0.8 |
| Granulocytes, %4-150 | 30.9 ± 4.6 | 6.9 ± 0.3 | 11.5 ± 1.8 |
| Lymphocytes, %4-150 | 53.0 ± 4.3 | 76.1 ± 1.3 | 68.3 ± 0.8 |
| B220+ lymphocytes, %4-150 | 18.3 ± 1.1 | 38.0 ± 1.3 | 42.2 ± 1.9 |
| Bone marrow4-151 | |||
| BFU-E | 19.2 ± 2.0 | 21.5 ± 2.9 | 19.2 ± 3.2 |
| CFU-GEMM | 11.5 ± 1.8 | 9.3 ± 1.3 | 10.2 ± 1.6 |
| HPP-CFC | 3.3 ± 0.4 | 3.0 ± 0.5 | 3.0 ± 0.4 |
| CFU-C | 25.0 ± 3.7 | 32.5 ± 2.9 | 21.8 ± 1.3 |
| Parameter . | Csf1op/Csf1op . | +/Csf1op . | Csf1op/Csf1op; TgC/+ . |
|---|---|---|---|
| Bone marrow cellularity, × 10−6cells per femur4-150 | 6.3 ± 0.9 | 19.8 ± 1.5 | 16.2 ± 0.6 |
| Splenic weight, g | 0.083 ± 0.05 | 0.097 ± 0.01 | 0.109 ± 0.03 |
| Splenic cellularity, × 10−9/g4-150 | 2.9 ± 0.6 | 1.5 ± 0.17 | 1.5 ± 0.1 |
| Blood cellularity | |||
| Total leukocytes, × 10−6/mL | 5.9 ± 1.1 | 5.0 ± 0.3 | 4.7 ± 0.6 |
| Monocytes, %4-150 | 0.9 ± 0.2 | 7.3 ± 0.8 | 8.1 ± 0.8 |
| Granulocytes, %4-150 | 30.9 ± 4.6 | 6.9 ± 0.3 | 11.5 ± 1.8 |
| Lymphocytes, %4-150 | 53.0 ± 4.3 | 76.1 ± 1.3 | 68.3 ± 0.8 |
| B220+ lymphocytes, %4-150 | 18.3 ± 1.1 | 38.0 ± 1.3 | 42.2 ± 1.9 |
| Bone marrow4-151 | |||
| BFU-E | 19.2 ± 2.0 | 21.5 ± 2.9 | 19.2 ± 3.2 |
| CFU-GEMM | 11.5 ± 1.8 | 9.3 ± 1.3 | 10.2 ± 1.6 |
| HPP-CFC | 3.3 ± 0.4 | 3.0 ± 0.5 | 3.0 ± 0.4 |
| CFU-C | 25.0 ± 3.7 | 32.5 ± 2.9 | 21.8 ± 1.3 |
At least three 6- to 8-week-old mice per experimental group. Data are expressed as means ± SEM. BFU-E and CFU-GEMM: 1.5 × 104 cells per plate (n = 6); HPP-CFC: 2.5 × 103 cells per plate (n = 6); and CFU-C: 1 × 104 cells per plate (n = 9).
BFU-E indicates burst-forming unit–erythroid; CFU-GEMM, colony-forming unit–granulocyte, erythroid, macrophage, megakaryocyte; HPP-CFC, colony-forming cells of high proliferative potential; CFU-C, colony-forming cells of low proliferative potential.
From FACS analyses (Figure 7). Differences betweenCsf1op/Csf1op; TgC/+ andCsf1op/Csf1op mice significant (P < .01) and differences betweenCsf1op/Csf1op; TgC/+ and+/Csf1op not significant (P > .05) by Student t test.
Differences not significant within assays (P ≥ .05) by Student t test.