Colony formation from 8.5 dpc YS and P-Sp cells (0.5 embryo equivalents) or their progenies cocultured with AGM-S3 cells
| Cell source . | Culture period, d . | No. of colonies . | ||||
|---|---|---|---|---|---|---|
| Granulocyte/ macrophage colonies . | Erythroid bursts . | Megakaryocyte colonies . | Hematopoietic mixed colonies . | Total . | ||
| 8.5 dpc YS | 0 | 7 ± 1 | 1 ± 1 | 1 ± 1 | 7 ± 3 | 16 ± 4 |
| 2 | 10 ± 2 | 16 ± 3 | 1 ± 1 | 9 ± 4 | 36 ± 9 | |
| 4 | 4 ± 3 | 2 ± 1 | 1 ± 1 | 5 ± 2 | 12 ± 4 | |
| 6 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| 10 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| 8.5 dpc P-Sp | 0 | 4 ± 2 | 0 ± 0 | 0 ± 1 | 2 ± 1 | 6 ± 3 |
| 2 | 0 ± 1 | 1 ± 1 | 0 ± 0 | 0 ± 0 | 1 ± 0 | |
| 4 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| 6 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| 10 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| Cell source . | Culture period, d . | No. of colonies . | ||||
|---|---|---|---|---|---|---|
| Granulocyte/ macrophage colonies . | Erythroid bursts . | Megakaryocyte colonies . | Hematopoietic mixed colonies . | Total . | ||
| 8.5 dpc YS | 0 | 7 ± 1 | 1 ± 1 | 1 ± 1 | 7 ± 3 | 16 ± 4 |
| 2 | 10 ± 2 | 16 ± 3 | 1 ± 1 | 9 ± 4 | 36 ± 9 | |
| 4 | 4 ± 3 | 2 ± 1 | 1 ± 1 | 5 ± 2 | 12 ± 4 | |
| 6 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| 10 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| 8.5 dpc P-Sp | 0 | 4 ± 2 | 0 ± 0 | 0 ± 1 | 2 ± 1 | 6 ± 3 |
| 2 | 0 ± 1 | 1 ± 1 | 0 ± 0 | 0 ± 0 | 1 ± 0 | |
| 4 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| 6 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
| 10 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | |
The number of colonies indicates the mean ± SD of triplicate cultures.