Table 1. T-cell proliferation and in... | American Society of Hematology

Table 1.

T-cell proliferation and in vitro anti–GPIIb-IIIa antibody production in response to recombinant GPIIb-IIIa fragments in 25 patients with immune thrombocytopenic purpura

Patients with ITP	Age/sex	Treatment	Anti–GPIIb-IIIa		Fragment-induced T-cell proliferation (SI)^*						Anti–GPIIb-IIIa antibody (OD₄₅₀)
Patients with ITP	Age/sex	Treatment	Plasma	Platelet eluates	IIbα18-259	IIbα244-575	IIbα566-841	IIIa22-262	IIIa254-462	IIIa708-762	GST	IIbα18-259	IIbα244-575	IIbα566-841	IIIa22-262	IIIa254-462	IIIa708-762
ITP1	53/F	cs, (sp)	−	+	3.50	—	—	—	—	—	NT
ITP2	51/M	None	−	+	6.80	—	3.98	—	—	3.90	NT
ITP3	42/F	None	+	+	—	—	—	4.23	—	—	NT
ITP4	60/F	cs	+	+	5.08	—	4.55	8.52	—	7.54	0.064	0.547^†	0.097	0.399^†	0.694^†	0.102	0.071
ITP5	29/F	None	−	+	17.98	—	3.73	11.36	—	3.85	NT
ITP6	72/F	None	−	+	—	—	17.20	—	—	—	NT
ITP7	51/F	(cs, sp)	−	+	5.34	—	—	—	4.00	—	NT
ITP8	56/M	cs, (sp)	+	+	3.29	—	—	—	—	—	0.037	0.317^†	0.030	0.034	0.043	0.037	0.022
ITP9	23/F	(cs)	−	+	3.42	7.57	3.39	5.68	—	—	0.039	0.243^†	0.099	0.219^†	0.549^†	0.044	0.036
ITP10	43/F	cs	+	+	—	—	—	8.58	—	—	0.089	0.079	0.086	0.094	0.428^†	0.088	0.079
ITP11	62/F	cs	+	+	6.58	—	—	—	—	—	0.057	0.671^†	0.056	0.049	0.087	0.054	0.029
ITP12	59/F	cs	−	−	3.90	—	—	—	—	—	NT
ITP13	56/F	None	+	+	4.75	—	—	4.07	—	—	0.034	0.279^†	0.024	0.051	0.605^†	0.028	0.026
ITP14	57/F	(cs, sp)	−	+	3.54	—	—	3.15	—	—	0.039	0.197^†	0.046	0.041	0.372^†	0.061	0.056
ITP15	49/F	None	+	+	—	5.32	—	5.08	—	—	0.069	0.049	0.072	0.054	0.397^†	0.069	0.039
ITP16	33/F	None	−	+	4.62	—	—	3.19	—	—	NT
ITP17	42/F	(cs)	+	+	—	—	—	3.68	—	—	NT
ITP18	60/M	cs	−	−	—	—	—	4.03	—	—	0.008	0.001	0.002	0.009	0.112^†	0.000	0.004
ITP19	46/F	cs	+	+	—	—	—	11.08	—	—	0.026	0.028	0.036	0.024	0.239^†	0.026	0.021
ITP20	71/F	cs, (sp, dz, vcr, az)	+	+	5.14	—	—	—	—	—	0.038	0.551^†	0.052	0.050	0.072	0.047	0.048
ITP21	62/F	cs	+	+	6.40	—	—	—	—	—	0.039	0.603^†	0.061	0.038	0.071	0.036	0.045
ITP22	54/F	cs, (sp)	−	−	—	5.70	—	5.06	—	—	NT
ITP23	53/F	cs	+	+	—	—	—	5.19	—	—	0.046	0.049	0.051	0.084	0.695^†	0.042	0.041
ITP24	21/F	(cs)	−	+	8.20	—	3.73	9.62	—	—	0.022	0.146^†	0.012	0.028	0.320^†	0.012	0.019
ITP25	54/F	(cs, sp, dz)	−	+	—	—	7.48	8.25	—	—	0.019	0.017	0.021	0.061	0.409^†	0.049	0.021

Patients with ITP	Age/sex	Treatment	Anti–GPIIb-IIIa		Fragment-induced T-cell proliferation (SI)^*						Anti–GPIIb-IIIa antibody (OD₄₅₀)
Patients with ITP	Age/sex	Treatment	Plasma	Platelet eluates	IIbα18-259	IIbα244-575	IIbα566-841	IIIa22-262	IIIa254-462	IIIa708-762	GST	IIbα18-259	IIbα244-575	IIbα566-841	IIIa22-262	IIIa254-462	IIIa708-762
ITP1	53/F	cs, (sp)	−	+	3.50	—	—	—	—	—	NT
ITP2	51/M	None	−	+	6.80	—	3.98	—	—	3.90	NT
ITP3	42/F	None	+	+	—	—	—	4.23	—	—	NT
ITP4	60/F	cs	+	+	5.08	—	4.55	8.52	—	7.54	0.064	0.547^†	0.097	0.399^†	0.694^†	0.102	0.071
ITP5	29/F	None	−	+	17.98	—	3.73	11.36	—	3.85	NT
ITP6	72/F	None	−	+	—	—	17.20	—	—	—	NT
ITP7	51/F	(cs, sp)	−	+	5.34	—	—	—	4.00	—	NT
ITP8	56/M	cs, (sp)	+	+	3.29	—	—	—	—	—	0.037	0.317^†	0.030	0.034	0.043	0.037	0.022
ITP9	23/F	(cs)	−	+	3.42	7.57	3.39	5.68	—	—	0.039	0.243^†	0.099	0.219^†	0.549^†	0.044	0.036
ITP10	43/F	cs	+	+	—	—	—	8.58	—	—	0.089	0.079	0.086	0.094	0.428^†	0.088	0.079
ITP11	62/F	cs	+	+	6.58	—	—	—	—	—	0.057	0.671^†	0.056	0.049	0.087	0.054	0.029
ITP12	59/F	cs	−	−	3.90	—	—	—	—	—	NT
ITP13	56/F	None	+	+	4.75	—	—	4.07	—	—	0.034	0.279^†	0.024	0.051	0.605^†	0.028	0.026
ITP14	57/F	(cs, sp)	−	+	3.54	—	—	3.15	—	—	0.039	0.197^†	0.046	0.041	0.372^†	0.061	0.056
ITP15	49/F	None	+	+	—	5.32	—	5.08	—	—	0.069	0.049	0.072	0.054	0.397^†	0.069	0.039
ITP16	33/F	None	−	+	4.62	—	—	3.19	—	—	NT
ITP17	42/F	(cs)	+	+	—	—	—	3.68	—	—	NT
ITP18	60/M	cs	−	−	—	—	—	4.03	—	—	0.008	0.001	0.002	0.009	0.112^†	0.000	0.004
ITP19	46/F	cs	+	+	—	—	—	11.08	—	—	0.026	0.028	0.036	0.024	0.239^†	0.026	0.021
ITP20	71/F	cs, (sp, dz, vcr, az)	+	+	5.14	—	—	—	—	—	0.038	0.551^†	0.052	0.050	0.072	0.047	0.048
ITP21	62/F	cs	+	+	6.40	—	—	—	—	—	0.039	0.603^†	0.061	0.038	0.071	0.036	0.045
ITP22	54/F	cs, (sp)	−	−	—	5.70	—	5.06	—	—	NT
ITP23	53/F	cs	+	+	—	—	—	5.19	—	—	0.046	0.049	0.051	0.084	0.695^†	0.042	0.041
ITP24	21/F	(cs)	−	+	8.20	—	3.73	9.62	—	—	0.022	0.146^†	0.012	0.028	0.320^†	0.012	0.019
ITP25	54/F	(cs, sp, dz)	−	+	—	—	7.48	8.25	—	—	0.019	0.017	0.021	0.061	0.409^†	0.049	0.021

SI indicates stimulation index; OD₄₅₀, optical density at 450 nm; NT, not tested; GST, glutathione S-transferase; cs, corticosteroids; sp, splenectomy; dz, danazol; vcr, vincristine; az, azathioprine. Previous treatment is shown in parentheses.

Negative results (SI < 3) are indicated by dashes.

†

Significant anti–GPIIb-IIIa antibody levels in culture supernatants.

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