Expression of protected p16 exon 2 fragment in polycythemia vera and normal erythroid colony-forming cells
| Exp no. . | Normal ECFCs . | PV ECFCs . | p16 density, healthy donors . | p16 density, patients with PV . | % increase in PV . |
|---|---|---|---|---|---|
| 1 | N7 | 1 073 | |||
| PV3 | 17 044 | 1 590 | |||
| PV6 | 5 863 | 550 | |||
| N9 | 1 437 | ||||
| PV4 | 5 834 | 410 | |||
| PV5 | 15 275 | 1 063 | |||
| 2 | N11 | 731 | |||
| PV1 | 6 085 | 845 | |||
| 3 | N2 | 630 | |||
| PV2 | 2 668 | 283 | |||
| 4 | N13 | 3 319 | |||
| PV8 | 16 173 | 387 | |||
| 5 | N12 | 2 290 | |||
| PV9 | 8 300 | 362 | |||
| 6 | N8 | 1 225 | |||
| PV7 | 3 478 | 284 | |||
| 7 | N14 | 1 280 | |||
| PV10 | 3 625 | 283 | |||
| N15 | 651 | ||||
| PV11 | 3 707 | 439 |
| Exp no. . | Normal ECFCs . | PV ECFCs . | p16 density, healthy donors . | p16 density, patients with PV . | % increase in PV . |
|---|---|---|---|---|---|
| 1 | N7 | 1 073 | |||
| PV3 | 17 044 | 1 590 | |||
| PV6 | 5 863 | 550 | |||
| N9 | 1 437 | ||||
| PV4 | 5 834 | 410 | |||
| PV5 | 15 275 | 1 063 | |||
| 2 | N11 | 731 | |||
| PV1 | 6 085 | 845 | |||
| 3 | N2 | 630 | |||
| PV2 | 2 668 | 283 | |||
| 4 | N13 | 3 319 | |||
| PV8 | 16 173 | 387 | |||
| 5 | N12 | 2 290 | |||
| PV9 | 8 300 | 362 | |||
| 6 | N8 | 1 225 | |||
| PV7 | 3 478 | 284 | |||
| 7 | N14 | 1 280 | |||
| PV10 | 3 625 | 283 | |||
| N15 | 651 | ||||
| PV11 | 3 707 | 439 |
The density of p16 exon 2 assayed by RNase protection assay was quantified with a laser scanning densitometer and normalized to the amount of the total RNA present in the lane by comparison with the housekeeping gene mRNA. The percentage increase in patients with PV was obtained using the density in PV divided by the normal control density in the same gel so that all conditions were the same.
ECFCs indicates erythroid colony-forming cells; PV, polycythemia vera.