Table 1. Summary of p53 abnormalities in... | American Society of Hematology

Table 1.

Summary of p53 abnormalities in splenic lymphoma with villous lymphocytes obtained by different techniques

Abnormality: Technique	Patients studied		Patients with p53 abnormalities
Abnormality: Technique	All	Untreated prior to testing	All	Untreated prior to testing
p53 deletion:
FISH	59	42	10 (17%)	8 (19%)
p53 protein expression:
Immunocytochemistry	35/59^*	22/42^*	3 (9%)^1-153	3 (13.6%)^1-153
Flow cytometry	20/35^†	15/22^†	2 (10%)	2 (13.3%)
p53 mutations:
Direct sequencing	9/11^‡	8/9^‡	2 (22%)	2 (25%)

Abnormality: Technique	Patients studied		Patients with p53 abnormalities
Abnormality: Technique	All	Untreated prior to testing	All	Untreated prior to testing
p53 deletion:
FISH	59	42	10 (17%)	8 (19%)
p53 protein expression:
Immunocytochemistry	35/59^*	22/42^*	3 (9%)^1-153	3 (13.6%)^1-153
Flow cytometry	20/35^†	15/22^†	2 (10%)	2 (13.3%)
p53 mutations:
Direct sequencing	9/11^‡	8/9^‡	2 (22%)	2 (25%)

FISH indicates fluorescence in situ hybridization.

Cytospins available in 35 of 59 (22 of 42 for untreated) patients in whom FISH was performed.

†

Cells for flow cytometry available in 20 of 35 (15 of 22 for untreated) analyzed by immunocytochemistry.

‡

DNA available in 9 of 11 (8 of 9 for untreated) patients with p53 deletion and/or p53 protein accumulation.

F1-153

Protein expression was found in 2 of 10 (2 of 8 for untreated) patients with a p53 deletion, one patient had protein expression without accompanying deletion and was only tested by immunocytochemistry.

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