Irradiated leukocytes co-infused with bone marrow cells enhance the engraftment in both myeloid and lymphoid lineages
| Irradiated leukocytes . | Mice* . | Donor-derived cells (FVB mice, H-2q) (%) . | Degree2-153 . | |
|---|---|---|---|---|
| Lymphocytes mean ± SEM†(range)‡ . | Granulocytes + monocytes mean ± SEM† (range)‡ . | |||
| None | 5/44 | 63 ± 15 (28-98) | 67 ± 12 (25-91) | 2/5 |
| FVB (H-2q) | 29/51 | 92 ± 3 (61-100) | 93 ± 3 (53-100) | 25/29 |
| BALB/c (H-2d) | 14/25 | 77 ± 7 (18-100) | 82 ± 6 (27-100) | 12/14 |
| C57BL/6 (H-2b) | 11/25 | 92 ± 3 (72-100) | 88 ± 3 (67-100) | 9/11 |
| PBMC | 12/23 | 90 ± 4 (43-100) | 90 ± 2 (76-100) | 10/12 |
| Irradiated leukocytes . | Mice* . | Donor-derived cells (FVB mice, H-2q) (%) . | Degree2-153 . | |
|---|---|---|---|---|
| Lymphocytes mean ± SEM†(range)‡ . | Granulocytes + monocytes mean ± SEM† (range)‡ . | |||
| None | 5/44 | 63 ± 15 (28-98) | 67 ± 12 (25-91) | 2/5 |
| FVB (H-2q) | 29/51 | 92 ± 3 (61-100) | 93 ± 3 (53-100) | 25/29 |
| BALB/c (H-2d) | 14/25 | 77 ± 7 (18-100) | 82 ± 6 (27-100) | 12/14 |
| C57BL/6 (H-2b) | 11/25 | 92 ± 3 (72-100) | 88 ± 3 (67-100) | 9/11 |
| PBMC | 12/23 | 90 ± 4 (43-100) | 90 ± 2 (76-100) | 10/12 |
Sublethally irradiated BALB/c (H-2d) recipient mice were grafted with 106 bone marrow cells from FVB (H-2q) mice alone or with irradiated leukocytes from different origins. The percentage of donor cells in lymphocytes and granulocytes + monocytes was determined by flow cytometry as described in the “Methods and materials” section and shown in Figure 2. PBMC, peripheral blood mononuclear cell.
Number of engrafted mice/number of analyzed mice at day 45 to day 50 post-BMT.
Data are from engrafted mice only of 3 to 5 independent experiments.
Data indicate the percentage range of donor cells in engrafted mice.
Number of mice with a full donor phenotype/number of engrafted mice.