Table 5. Determination of competent... | American Society of Hematology

Table 5.

Determination of competent virus via messenger RNA detection and tissue culture

Subject^5-150	Location	Day 0				Day 5				Tissue culture
Subject^5-150	Location	CMV (GE/mL)	RNA IE	RNA POL	RNA late	CMV (GE/mL)	RNA IE	RNA POL	RNA late	3 d	10 d
H1	Denver	1 000 000			POS	1 000 000	POS	POS		POS	POS
N2	Denver	1 000 000	POS	POS	POS	1 000 000	POS	POS	POS	POS	POS
O2	Denver	110 800	POS			100
Q2	Denver	110 000	POS			351 400
D	Norfolk	105 000	POS			468 200	POS				POS
J1	Denver	97 300	POS			426 300
M1	Denver	93 000				105 200
B1	Norfolk	64 900	POS	POS		506 200	POS				POS
R1	Denver	60 100				73 100
K1	Norfolk	49 000				52 800
O1	Denver	41 200				100
A1	Norfolk	36 100				53 300
F	Norfolk	33 100				41 600
E	Norfolk	26 800				69 100
A2	Norfolk	24 200				62 800
P1	Denver	16 700				56 100	POS
C1	Norfolk	16 600				31 900
Q1	Denver	13 400				47 900
J2	Denver	12 300				106 100
C2	Norfolk	11 700				34 300
B2	Norfolk	11 200				24 700
R2	Denver	9 400				16 800
G	Denver	5 700				21 800
N1	Denver	4 200	POS			31 300
H2	Denver	4 100				105 500
L1	Denver	3 200				19 500
L2	Denver	2 700				23 200
I	Denver	2 600				3 800
S	Denver	2 000				10 200
M2	Denver	1 600				4 700
K2	Norfolk	1 382				10 600
P2	Denver	100				3 200

Subject^5-150	Location	Day 0				Day 5				Tissue culture
Subject^5-150	Location	CMV (GE/mL)	RNA IE	RNA POL	RNA late	CMV (GE/mL)	RNA IE	RNA POL	RNA late	3 d	10 d
H1	Denver	1 000 000			POS	1 000 000	POS	POS		POS	POS
N2	Denver	1 000 000	POS	POS	POS	1 000 000	POS	POS	POS	POS	POS
O2	Denver	110 800	POS			100
Q2	Denver	110 000	POS			351 400
D	Norfolk	105 000	POS			468 200	POS				POS
J1	Denver	97 300	POS			426 300
M1	Denver	93 000				105 200
B1	Norfolk	64 900	POS	POS		506 200	POS				POS
R1	Denver	60 100				73 100
K1	Norfolk	49 000				52 800
O1	Denver	41 200				100
A1	Norfolk	36 100				53 300
F	Norfolk	33 100				41 600
E	Norfolk	26 800				69 100
A2	Norfolk	24 200				62 800
P1	Denver	16 700				56 100	POS
C1	Norfolk	16 600				31 900
Q1	Denver	13 400				47 900
J2	Denver	12 300				106 100
C2	Norfolk	11 700				34 300
B2	Norfolk	11 200				24 700
R2	Denver	9 400				16 800
G	Denver	5 700				21 800
N1	Denver	4 200	POS			31 300
H2	Denver	4 100				105 500
L1	Denver	3 200				19 500
L2	Denver	2 700				23 200
I	Denver	2 600				3 800
S	Denver	2 000				10 200
M2	Denver	1 600				4 700
K2	Norfolk	1 382				10 600
P2	Denver	100				3 200

Peripheral blood samples from 7 Norfolk subjects and 12 Denver on the day of CMV-DNAemia were further evaluated by RT-PCR forIE, POL, and late gene products on days 0 and 5 of culture. Tissue culture was performed by incubation of fibroblast with peripheral blood white cells for 18 hours and staining of fibroblast with monoclonal antibodies to IE protein and RT-PCR for IE gene products at days 3 and 10 after incubation.

See Table 1 for other abbreviations.

F5-150

Number indicates first or second sample for subjects having samples from 2 separate dates tested.

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