Generation of dendritic cell progeny on day 15 in spleen and thymus from common lymphoid progenitors, common myeloid progenitors, early thymocyte progenitors, pro-T cells, and granulocyte-macrophage progenitors after transplantation into lethally irradiated animals
Progenitor population . | No. IV-transplanted progenitors . | Progenitor-derived DC . | Progenitor no. (%) in 4 long bones resp. thymus3-150 . | Extrapolated DC yield3-150 . | ||
---|---|---|---|---|---|---|
Thymus . | Spleen . | Thymus . | Spleen . | |||
CLP | 1 × 104 | 8930 | 68 770 | 2 × 104 (0.02) | 17 860 | 137 540 |
CMP | 2 × 104 | 1990 | 95 010 | 20 × 104 (0.2) | 19 900 | 950 100 |
GMP | 4 × 104 | 280 | 12 500 | 40 × 104 (0.4) | 2800 | 125 000 |
TP | 2 × 104 | 2350 | 25 240 | 3 × 104 (0.02) | 3525 | 37 860 |
Pro-T | 3 × 104 | 500 | 5430 | 6 × 104 (0.04) | 1000 | 10 860 |
Progenitor population . | No. IV-transplanted progenitors . | Progenitor-derived DC . | Progenitor no. (%) in 4 long bones resp. thymus3-150 . | Extrapolated DC yield3-150 . | ||
---|---|---|---|---|---|---|
Thymus . | Spleen . | Thymus . | Spleen . | |||
CLP | 1 × 104 | 8930 | 68 770 | 2 × 104 (0.02) | 17 860 | 137 540 |
CMP | 2 × 104 | 1990 | 95 010 | 20 × 104 (0.2) | 19 900 | 950 100 |
GMP | 4 × 104 | 280 | 12 500 | 40 × 104 (0.4) | 2800 | 125 000 |
TP | 2 × 104 | 2350 | 25 240 | 3 × 104 (0.02) | 3525 | 37 860 |
Pro-T | 3 × 104 | 500 | 5430 | 6 × 104 (0.04) | 1000 | 10 860 |
IV indicates intravenous; CLP, common lymphoid progenitor; CMP, common myeloid progenitor; GMP, granulocyte-macrophage progenitor; TP, thymocyte progenitor.
Results are given as means of 2 experiments, each involving 3 to 5 animals. For each measurement point, 106 cells were analyzed by FACS.
Progenitor number and % refer to total cell numbers in femurs and tibias of both legs (CLP, CMP, GMP) or in the thymus (TP, Pro-T) of a 6-8 week old animal, respectively.