Levels of various chemokines detected by enzyme-linked immunosorbent assay (pg/mL) in media conditioned by normal human hematopoietic cells (n = 5)
| Factor detected . | CD34+ cells BM . | CFU-GM– derived . | BFU-E– derived . | CFU-Meg– derived . | Stroma . |
|---|---|---|---|---|---|
| MIP-1α | 2012 ± 477 | bsd4-150 | bsd | bsd | 350 ± 42 |
| MIP-1β | 1309 ± 317 | bsd | bsd | bsd | —4-151 |
| RANTES | 174 ± 29 | bsd | bsd | 2227 ± 431 | 472 ± 63 |
| IL-8 | 103 ± 21 | — | — | 823 ± 54 | 397 ± 45 |
| PF-4 | 130 ± 25 | bsd | bsd | 9597 ± 862 | bsd |
| MCP-1 | bsd | bsd | bsd | bsd | 4221 ± −889 |
| Factor detected . | CD34+ cells BM . | CFU-GM– derived . | BFU-E– derived . | CFU-Meg– derived . | Stroma . |
|---|---|---|---|---|---|
| MIP-1α | 2012 ± 477 | bsd4-150 | bsd | bsd | 350 ± 42 |
| MIP-1β | 1309 ± 317 | bsd | bsd | bsd | —4-151 |
| RANTES | 174 ± 29 | bsd | bsd | 2227 ± 431 | 472 ± 63 |
| IL-8 | 103 ± 21 | — | — | 823 ± 54 | 397 ± 45 |
| PF-4 | 130 ± 25 | bsd | bsd | 9597 ± 862 | bsd |
| MCP-1 | bsd | bsd | bsd | bsd | 4221 ± −889 |
Cells were cultured for 24 hours in serum-free medium at a concentration of 1 × 106/mL medium.
See footnotes to Tables 1, 2, and 3 for abbreviations.
The sensitivities of the enzyme-linked immunosorbent assays (pg/mL) were as follows: MIP-1α > 31, MIP-1β > 31, MCP-1 > 31, PF-4 > 10, IL-8 > 1.
A dash indicates not done.