Reverse transcriptase–polymerase chain reaction analysis of highly purified CD34+ cells (n = 3 for bone marrow and n = 5 for peripheral blood), myeloblasts, erythroblasts, megakaryoblasts, and bone marrow–derived fibroblasts (n = 3 for each) for expression of various growth factors, cytokines, and chemokines
| . | CD34+ BM . | CD34+PB . | CFU-GM– derived . | BFU-E– derived . | CFU-Meg– derived . | CFU-F– derived . |
|---|---|---|---|---|---|---|
| KL | + | + | + | + | + | + |
| FLT3 L | + | + | + | + | − | + |
| M-CSF | − | − | + | + | + | + |
| FGF-2 | + | + | + | + | + | + |
| VEGF | + | + | + | + | + | + |
| HGF | + | + | − | + | + | + |
| IGF-1 | + | + | − | + | + | + |
| NGF-β | − | − | − | − | − | + |
| IL-1β | + | + | − | − | − | − |
| IL-3 | − | − | − | − | − | + |
| IL-6 | − | − | − | − | − | + |
| IL-16 | + | + | + | + | + | + |
| EPO | − | − | − | − | − | − |
| TPO | + | + | + | + | + | + |
| G-CSF | − | − | − | − | − | + |
| GM-CSF | − | + | − | − | + | + |
| TNF-α | + | + | + | + | + | + |
| IFN-α | + | + | + | + | + | + |
| Fas-L | + | + | + | + | + | + |
| MIP-1α | + | + | + | + | + | − |
| MIP-1β | + | + | + | − | + | + |
| RANTES | + | + | + | − | + | + |
| MCP-1 | − | − | − | − | − | + |
| MCP-2 | − | + | + | + | + | + |
| MCP-3 | + | + | + | − | + | + |
| MCP-4 | + | + | + | + | + | + |
| IL-8 | + | + | − | − | + | − |
| IP-10 | + | + | + | + | + | − |
| MDC | + | + | − | − | − | − |
| SDF-1 | − | − | − | − | − | + |
| PF-4 | + | + | + | + | + | + |
| . | CD34+ BM . | CD34+PB . | CFU-GM– derived . | BFU-E– derived . | CFU-Meg– derived . | CFU-F– derived . |
|---|---|---|---|---|---|---|
| KL | + | + | + | + | + | + |
| FLT3 L | + | + | + | + | − | + |
| M-CSF | − | − | + | + | + | + |
| FGF-2 | + | + | + | + | + | + |
| VEGF | + | + | + | + | + | + |
| HGF | + | + | − | + | + | + |
| IGF-1 | + | + | − | + | + | + |
| NGF-β | − | − | − | − | − | + |
| IL-1β | + | + | − | − | − | − |
| IL-3 | − | − | − | − | − | + |
| IL-6 | − | − | − | − | − | + |
| IL-16 | + | + | + | + | + | + |
| EPO | − | − | − | − | − | − |
| TPO | + | + | + | + | + | + |
| G-CSF | − | − | − | − | − | + |
| GM-CSF | − | + | − | − | + | + |
| TNF-α | + | + | + | + | + | + |
| IFN-α | + | + | + | + | + | + |
| Fas-L | + | + | + | + | + | + |
| MIP-1α | + | + | + | + | + | − |
| MIP-1β | + | + | + | − | + | + |
| RANTES | + | + | + | − | + | + |
| MCP-1 | − | − | − | − | − | + |
| MCP-2 | − | + | + | + | + | + |
| MCP-3 | + | + | + | − | + | + |
| MCP-4 | + | + | + | + | + | + |
| IL-8 | + | + | − | − | + | − |
| IP-10 | + | + | + | + | + | − |
| MDC | + | + | − | − | − | − |
| SDF-1 | − | − | − | − | − | + |
| PF-4 | + | + | + | + | + | + |
CFU-GM indicates colony-forming unit–granulocyte-macrophage; BFU-E, burst forming unit–erythroid; CFU-Meg, colony-forming unit–megakaryocyte; CFU-F, CFU-fibroblasts. For other abbreviations, see footnote to Table 1.