Sequences of the primers used in reverse transcriptase–polymerase chain reaction studies
| Factor detected . | Sense primer . | Antisense primer . |
|---|---|---|
| KL | GAC AGC TTG ACT GAT CTT CTG GAC | ACT GCT GTC ATT CCT AAG GGA GCT |
| FLT3 L | AAC AAC CTA TCT CCT CCT GCT | GGC ACA TTT GGT GAC AAA GTG |
| M-CSF | TGT TGT TGG TCT GTC TCC TGG | TTC TCC AGC AAC TGG AGA GGT |
| FGF-2 | GGC CAC TTC AAG GAC CCC AAG | TCA GCT CTT AGC AGA CA |
| VEGF | TCG GGC CTC CGA AAC CAT GA | CCT GGT GAG AGA TCT GGT TC |
| HGF | GAG GGA CAT AAG AAA AGA AGA | GTG TGG TAT CAT GGA ACT CCA |
| IGF-1 | ACA TCT CCC ATC TCT CTG GAT TTC CTT TTG | CCC TCT ACT TGC GTT CTT CAA ATG TAC TTC |
| NGF-β | ACA GTT TTA CCA AGG GAG CAG | ATG ATG ACC GCT TGC TCC TGT |
| IL-1β | AGT GAA ATG ATG GCT TAT TAC | TAC ACT CTC CAG CTG TAG AGT |
| IL-3 | GCT CCC ATG ACC CAG ACA ACG TCC | CAG ATA GAA CGT CAG TTT CCT CCG |
| IL-6 | AAC TCC TTC TCC ACA AGC GCC TT | CCG AAG AGC CCT CAG GCT GG |
| IL-16 | CAC TCC CAA AGT TTA CAA GTC | GAG ATT CCT CAG CCT GTG TTG |
| EPO | ATC ACG ACG GGC TGT GCT GAA CAC | GGG AGA TGG CTT CCT TCT GGG CTC |
| TPO | TGC TCC GAG GAA AGG TGC GTT | GGG AAG AGC GTA TAC TGT CCA |
| G-CSF | GCC AGC TCC CTG CCC CAG AGC TTC | GTG TGT CCA AGG TGG GAC CCA ACT |
| GM-CSF | CAG GAG GCC CGG CGT CTC CTG AAC | ACA AGC AGA AAG TCC TTC AGG TTC |
| TNF-α | CAG AGG GAA GAG TTC CCC AG | TTG ACC TTG GTC TGG TAG GAG |
| IFN-α | CTT TGC TTT ACT GAT GGT CCT G | GAT CTC ATG ATT TCT GCT CTG |
| Fas-L | CAA GTC CAA CTC AAG GTC CAT GCC | CAG AGA GAG CTC AGA TAC GTT GAC |
| MIP-1α | CCT TGC TGT CCT CCT CTG CAC | CAC TCA GCT CCA GGT CGC TGA |
| MIP-1β | TGT CCT CCT CAT GCT AGT AG | GTA CTC CTG GCC CAG GAT TC |
| RANTES | TCA TTG CTA CTG CCC TCT GCG | CTC ATC TCC AAA GAG TTG ATG |
| MCP-1 | GCT GCT CAT AGC AGC CAC CTT | ACA ATG GTC TTG AAG ATC ACA |
| MCP-2 | TGC TGA AGC TCA CAC CCT TGC | TGG TCC AGA TGC TTC ATG GAA |
| MCP-3 | ACT GAA CTG AAA ACA AGC CA | TCC AAG GCT TTA TGT TCA AA |
| MCP-4 | AGC TGG AGT ACG TGA AAT GA | AGC TCA TAG TGG AAG GGA AG |
| IL-8 | GCT TTC TGA TGG AAG AGA GCT | CTT GGA TAC CAC AGA GAA TGA |
| IP-10 | CCT GCT TCA AAT ATT TCC CT | CCT TCC TGT ATG TGT TTG GA |
| MDC | GCT CGC CTA CAG ACT GCA CTC CTG | GCT TAT TGA GAA TCA TCT TCA CCC AGG |
| SDF-1 | AAC GCC AAG GTC GTG GTC GTG CTG | CAC ATG TTG AAC CTC TTG TTT AAA AGC |
| PF-4 | GTC CAG TGG CAC CCT CTT GA | AAT TGA CAT TTA GGC AGC TGA |
| Factor detected . | Sense primer . | Antisense primer . |
|---|---|---|
| KL | GAC AGC TTG ACT GAT CTT CTG GAC | ACT GCT GTC ATT CCT AAG GGA GCT |
| FLT3 L | AAC AAC CTA TCT CCT CCT GCT | GGC ACA TTT GGT GAC AAA GTG |
| M-CSF | TGT TGT TGG TCT GTC TCC TGG | TTC TCC AGC AAC TGG AGA GGT |
| FGF-2 | GGC CAC TTC AAG GAC CCC AAG | TCA GCT CTT AGC AGA CA |
| VEGF | TCG GGC CTC CGA AAC CAT GA | CCT GGT GAG AGA TCT GGT TC |
| HGF | GAG GGA CAT AAG AAA AGA AGA | GTG TGG TAT CAT GGA ACT CCA |
| IGF-1 | ACA TCT CCC ATC TCT CTG GAT TTC CTT TTG | CCC TCT ACT TGC GTT CTT CAA ATG TAC TTC |
| NGF-β | ACA GTT TTA CCA AGG GAG CAG | ATG ATG ACC GCT TGC TCC TGT |
| IL-1β | AGT GAA ATG ATG GCT TAT TAC | TAC ACT CTC CAG CTG TAG AGT |
| IL-3 | GCT CCC ATG ACC CAG ACA ACG TCC | CAG ATA GAA CGT CAG TTT CCT CCG |
| IL-6 | AAC TCC TTC TCC ACA AGC GCC TT | CCG AAG AGC CCT CAG GCT GG |
| IL-16 | CAC TCC CAA AGT TTA CAA GTC | GAG ATT CCT CAG CCT GTG TTG |
| EPO | ATC ACG ACG GGC TGT GCT GAA CAC | GGG AGA TGG CTT CCT TCT GGG CTC |
| TPO | TGC TCC GAG GAA AGG TGC GTT | GGG AAG AGC GTA TAC TGT CCA |
| G-CSF | GCC AGC TCC CTG CCC CAG AGC TTC | GTG TGT CCA AGG TGG GAC CCA ACT |
| GM-CSF | CAG GAG GCC CGG CGT CTC CTG AAC | ACA AGC AGA AAG TCC TTC AGG TTC |
| TNF-α | CAG AGG GAA GAG TTC CCC AG | TTG ACC TTG GTC TGG TAG GAG |
| IFN-α | CTT TGC TTT ACT GAT GGT CCT G | GAT CTC ATG ATT TCT GCT CTG |
| Fas-L | CAA GTC CAA CTC AAG GTC CAT GCC | CAG AGA GAG CTC AGA TAC GTT GAC |
| MIP-1α | CCT TGC TGT CCT CCT CTG CAC | CAC TCA GCT CCA GGT CGC TGA |
| MIP-1β | TGT CCT CCT CAT GCT AGT AG | GTA CTC CTG GCC CAG GAT TC |
| RANTES | TCA TTG CTA CTG CCC TCT GCG | CTC ATC TCC AAA GAG TTG ATG |
| MCP-1 | GCT GCT CAT AGC AGC CAC CTT | ACA ATG GTC TTG AAG ATC ACA |
| MCP-2 | TGC TGA AGC TCA CAC CCT TGC | TGG TCC AGA TGC TTC ATG GAA |
| MCP-3 | ACT GAA CTG AAA ACA AGC CA | TCC AAG GCT TTA TGT TCA AA |
| MCP-4 | AGC TGG AGT ACG TGA AAT GA | AGC TCA TAG TGG AAG GGA AG |
| IL-8 | GCT TTC TGA TGG AAG AGA GCT | CTT GGA TAC CAC AGA GAA TGA |
| IP-10 | CCT GCT TCA AAT ATT TCC CT | CCT TCC TGT ATG TGT TTG GA |
| MDC | GCT CGC CTA CAG ACT GCA CTC CTG | GCT TAT TGA GAA TCA TCT TCA CCC AGG |
| SDF-1 | AAC GCC AAG GTC GTG GTC GTG CTG | CAC ATG TTG AAC CTC TTG TTT AAA AGC |
| PF-4 | GTC CAG TGG CAC CCT CTT GA | AAT TGA CAT TTA GGC AGC TGA |
KL indicates kit ligand; FLT3 L, FLT ligand; M-CSF, macrophage colony-stimulating factor; FGF, fibroblast growth factor; VEGF, vascular endothelial growth factor; HGF, hepatocyte growth factor; IGF, insulin-like growth factor; NGF, nerve growth factor; IL, interleukin; EPO, erythropoietin; TPO, thrombopoietin; G-CSF, granulocyte-colony stimulating factor; GM-CSF, granulocyte-macrophage colony stimulating factor; TNF, tumor necrosis factor; IFN, interferon; Fas-L, Fas ligand; MIP, macrophage inflammatory protein; MCP, monocyte chemoattractant protein; SDF, stromal cell–derived factor; PF, platelet factor.