Loss of sensitivity of splenic CFU-C to Fas-induced apoptosis after in vivo depletion of NK cells
| . | CFU-C/106 cells . | |
|---|---|---|
| Total spleen cells . | NK1.1-depleted . | |
| Medium | 60.7 ± 3.2 | 50.6 ± 3.3 |
| IFN-γ | 74.4 ± 7.0 | 59.3 ± 5.9 |
| Anti-Fas mAb | 65.6 ± 6.6 | 57.8 ± 3.7 |
| Anti-Fas mAb + IFN-γ | 31.1 ± 2.5* | 54.4 ± 5.1NS |
| . | CFU-C/106 cells . | |
|---|---|---|
| Total spleen cells . | NK1.1-depleted . | |
| Medium | 60.7 ± 3.2 | 50.6 ± 3.3 |
| IFN-γ | 74.4 ± 7.0 | 59.3 ± 5.9 |
| Anti-Fas mAb | 65.6 ± 6.6 | 57.8 ± 3.7 |
| Anti-Fas mAb + IFN-γ | 31.1 ± 2.5* | 54.4 ± 5.1NS |
Six-week-old C57BL/6-β2m−/− mice (chosen for their lack of NKT cells) received 2 intraperitoneal injections of 0.5 mg NK1.1 mAb per mouse 1 day and 2 hours before death compared with mice injected with isotype control. Data are means ± SEM from 3 individual mice.
NS, not significant in comparison with control.
P < .001.