Equilibrium dissociation constants for125I–fibroblast growth factor binding to α2M and α2M*
| Labeled ligand . | Kd (μM) . | |
|---|---|---|
| α2M . | α2M* . | |
| FGF-1 | 5.02 ± 3.01 | 0.59 ± 0.49 |
| FGF-2 | 0.82 ± 0.61 | 0.103 ± 0.037 |
| FGF-4 | 1.16 ± 0.50 | 0.34 ± 0.19 |
| FGF-5 | — | — |
| FGF-6 | 1.83 ± 1.03 | 0.332 ± .042 |
| FGF-7 | — | — |
| FGF-9 | — | — |
| FGF-10 | — | — |
| Labeled ligand . | Kd (μM) . | |
|---|---|---|
| α2M . | α2M* . | |
| FGF-1 | 5.02 ± 3.01 | 0.59 ± 0.49 |
| FGF-2 | 0.82 ± 0.61 | 0.103 ± 0.037 |
| FGF-4 | 1.16 ± 0.50 | 0.34 ± 0.19 |
| FGF-5 | — | — |
| FGF-6 | 1.83 ± 1.03 | 0.332 ± .042 |
| FGF-7 | — | — |
| FGF-9 | — | — |
| FGF-10 | — | — |
Binding was performed as described in “Materials and methods.” The binding of 125I-FGF to α2M or α2M* was quantitated by measuring the α2M- or α2M*-associated radioactivity by Phosphorimager. The binding values were determined from the mean of 3 (FGF-1 and FGF-6) or 4 (FGF-2 and FGF-4) independent experiments ± SD.
Kd indicates equilibrium dissociation constant; FGF, fibroblast growth factor. “—” indicates that these FGFs were investigated to determine if they bound to α2M or α2M* but that specific binding of these FGFs to α2M or α2M* was not detected (Figure 1).