PCR primers used in this study
| Primer . | Sequence . | Comment . |
|---|---|---|
| cDNA 165 forward | GGCGGGGGTGGTGCTGGCTC | cDNA amplification outer primers |
| cDNA 1232 reverse | GGTGGAGAAAAGGAGCTTCCAG | |
| cDNA 180 forward | GGTGGAGAAAAGGAGCTTCCAG | cDNA amplification nested primers |
| cDNA 1174 reverse | GTTATACAAAGGGAACACTTCGAGAG | |
| cDNA 315 forward | TATCAAAGGAGGAGCTGCCAAAC | cDNA sequencing |
| cDNA 443 reverse | AATAGCGTAGTATTTTTCCTTTAGTTGC | cDNA sequencing |
| cDNA 452 forward | GAATGTAGAAAAAAGTTATTGCAACTAAAG | cDNA sequencing |
| cDNA 552 forward | TATACTAAATCACATGGTTTGCTTGTTC | cDNA sequencing |
| cDNA 705 forward | GAATCGGCGATGTACTAGAGGAAG | cDNA sequencing |
| cDNA 734 reverse | ATAAAATTGGACACAACTTTGACATTG | cDNA sequencing |
| cDNA 849 forward | ATGATGGTGCCTTGAGGAATACAG | cDNA sequencing |
| cDNA 928 reverse | ATGTGCTCAACATTGGCCACT | cDNA sequencing |
| Exon 10 forward (with cDNA 1232 reverse) | TTGCCCAAGAGATCTAACAACGAG | Genomic PCR exon 10 |
| Exon 9 forward | AATATGATGAAATAATAGTGACATCAATTAAG | Genomic PCR exon 9 |
| Exon 9 reverse | CATCACTACACTCTAGCCTCGGTAAC | |
| Exon 8 forward | TGAAATGAGAATATGAGTGAGCCATAG | Genomic PCR exon 8 |
| Exon 8 reverse | TATCGGCTTGGCCTAATTTCTG | |
| Exon 7 forward | ATGCTTTGGAATAAAGATAATAATTTTAG | Genomic PCR exon 7 |
| Exon 7 reverse | TTTAAAAAGTACAACTGACTACATAAATAGC | |
| Exon 6 forward | TTTATTGTTGGTTTTTACTCATCTGAAAG | Genomic PCR exon 6 |
| Exon 6 reverse | TTATTAATCTGTTTGTTTGCAATACAGG | |
| Codon 92 mismatch reverse (with exon 6 forward) | GTAAGAACAGGATCAACTTCAATAGCTTA | Mismatch primer creates a DdeI site in the presence of the codon 92 |
| (TAC) polymorphism. The mismatch is underlined. | ||
| Pseu 226 forward (with cDNA 1232 reverse) | CAAGAACCCTACAAGAGTAGAAGAAATG | PCR of pseudogene from genomic DNA |
| Pseu 902 reverse (with cDNA 165 forward) | CATCCACTCTATCATTTAGATATCCAAC | PCR of pseudogene from genomic DNA |
| Primer . | Sequence . | Comment . |
|---|---|---|
| cDNA 165 forward | GGCGGGGGTGGTGCTGGCTC | cDNA amplification outer primers |
| cDNA 1232 reverse | GGTGGAGAAAAGGAGCTTCCAG | |
| cDNA 180 forward | GGTGGAGAAAAGGAGCTTCCAG | cDNA amplification nested primers |
| cDNA 1174 reverse | GTTATACAAAGGGAACACTTCGAGAG | |
| cDNA 315 forward | TATCAAAGGAGGAGCTGCCAAAC | cDNA sequencing |
| cDNA 443 reverse | AATAGCGTAGTATTTTTCCTTTAGTTGC | cDNA sequencing |
| cDNA 452 forward | GAATGTAGAAAAAAGTTATTGCAACTAAAG | cDNA sequencing |
| cDNA 552 forward | TATACTAAATCACATGGTTTGCTTGTTC | cDNA sequencing |
| cDNA 705 forward | GAATCGGCGATGTACTAGAGGAAG | cDNA sequencing |
| cDNA 734 reverse | ATAAAATTGGACACAACTTTGACATTG | cDNA sequencing |
| cDNA 849 forward | ATGATGGTGCCTTGAGGAATACAG | cDNA sequencing |
| cDNA 928 reverse | ATGTGCTCAACATTGGCCACT | cDNA sequencing |
| Exon 10 forward (with cDNA 1232 reverse) | TTGCCCAAGAGATCTAACAACGAG | Genomic PCR exon 10 |
| Exon 9 forward | AATATGATGAAATAATAGTGACATCAATTAAG | Genomic PCR exon 9 |
| Exon 9 reverse | CATCACTACACTCTAGCCTCGGTAAC | |
| Exon 8 forward | TGAAATGAGAATATGAGTGAGCCATAG | Genomic PCR exon 8 |
| Exon 8 reverse | TATCGGCTTGGCCTAATTTCTG | |
| Exon 7 forward | ATGCTTTGGAATAAAGATAATAATTTTAG | Genomic PCR exon 7 |
| Exon 7 reverse | TTTAAAAAGTACAACTGACTACATAAATAGC | |
| Exon 6 forward | TTTATTGTTGGTTTTTACTCATCTGAAAG | Genomic PCR exon 6 |
| Exon 6 reverse | TTATTAATCTGTTTGTTTGCAATACAGG | |
| Codon 92 mismatch reverse (with exon 6 forward) | GTAAGAACAGGATCAACTTCAATAGCTTA | Mismatch primer creates a DdeI site in the presence of the codon 92 |
| (TAC) polymorphism. The mismatch is underlined. | ||
| Pseu 226 forward (with cDNA 1232 reverse) | CAAGAACCCTACAAGAGTAGAAGAAATG | PCR of pseudogene from genomic DNA |
| Pseu 902 reverse (with cDNA 165 forward) | CATCCACTCTATCATTTAGATATCCAAC | PCR of pseudogene from genomic DNA |
5′-ends of primers are numbered according to the complementary DNA (cDNA) sequence in Figure 2.