Table 4. Engraftment in the fetal sheep... | American Society of Hematology

Table 4.

Engraftment in the fetal sheep model

Cell dose	1° Recipient						2° Recipient				3° Recipient
	Group	N	% Engraftment		CD45⁺× 10⁴^4-150	% CD34⁺	2° Recipient				3° Recipient
	Group	N	2 mo^‡	6 mo^4-153	CD45⁺× 10⁴^4-150	% CD34⁺	Group	N	% Engraftment^‡	MNC × 10⁶^4-151	Group	N	% Engraftment^‡
1 × 10⁵	I	4	4.8 ± 0.7	4.1 ± 0.5	1.9	17.2	IV	3	5.2	5.1	VII	3	4.7
D7 cultured	II	4	6.3 ± 1.4	4.3 ± 0.9	2.3	13.9	V	3	4.4	3.9	VIII	3	0.80
D14 cultured	III	4	5.6 ± 0.5	0.2 ± 0.2	1.7	7.2	VI	3	< 0.05	4.2	IX	3	0
5 × 10⁴	X	4	3.7	ND	3.3	11.3
D7 cultured	XI	4	5.4	ND	1.8	12.4
D14 cultured	XII	4	1.7	ND	1.8	5.3

Cell dose	1° Recipient						2° Recipient				3° Recipient
	Group	N	% Engraftment		CD45⁺× 10⁴^4-150	% CD34⁺	2° Recipient				3° Recipient
	Group	N	2 mo^‡	6 mo^4-153	CD45⁺× 10⁴^4-150	% CD34⁺	Group	N	% Engraftment^‡	MNC × 10⁶^4-151	Group	N	% Engraftment^‡
1 × 10⁵	I	4	4.8 ± 0.7	4.1 ± 0.5	1.9	17.2	IV	3	5.2	5.1	VII	3	4.7
D7 cultured	II	4	6.3 ± 1.4	4.3 ± 0.9	2.3	13.9	V	3	4.4	3.9	VIII	3	0.80
D14 cultured	III	4	5.6 ± 0.5	0.2 ± 0.2	1.7	7.2	VI	3	< 0.05	4.2	IX	3	0
5 × 10⁴	X	4	3.7	ND	3.3	11.3
D7 cultured	XI	4	5.4	ND	1.8	12.4
D14 cultured	XII	4	1.7	ND	1.8	5.3

Fetal sheep were transplanted with fresh or ex vivo–cultured UCB CD34⁺ cells from multiple donors (group I-III) or a single donor (group X-XII). After 2 or 6 months, marrow was obtained and the frequency of human CD45⁺ cells determined by flow cytometry. As described in “Materials and methods,” CD45⁺ cells or bone marrow mononuclear cells (BMMNC) were then transferred to secondary and tertiary recipients, respectively. These secondary and tertiary recipients were killed after 2 months and human cell engraftment determined. Results are derived from a single experiment.

F4-150

Total number of CD45⁺ cells recovered from 2 fetal sheep. These cells were split and given to 3 secondary recipients.

F4-151

Total number of BMMNC cells recovered from 3 secondary recipients. These cells were split and given to 3 tertiary recipients.

‡

Engraftment (mean ± SEM) of 2 fetal sheep killed at 2 months after transplantation.

F4-153

Engraftment (mean ± SEM) of 2 sheep at 6 months after transplantation.

ND indicates not determined.

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