CD34−CD38−Lin−cells demonstrate lower SDF-1–induced migration compared to CD34+CD38− cells
Cell fraction . | CD34+CD38−†(%) . | CD34−CD38−† (%) . |
---|---|---|
Enriched cells before migration‡ | 36.4 | 12.1 |
Spontaneously migrating cells | 12.3 | 17.5 |
Cells migrating to SDF-1 | 52.4 | 8.0 |
Cell fraction . | CD34+CD38−†(%) . | CD34−CD38−† (%) . |
---|---|---|
Enriched cells before migration‡ | 36.4 | 12.1 |
Spontaneously migrating cells | 12.3 | 17.5 |
Cells migrating to SDF-1 | 52.4 | 8.0 |
Data represent percentage of subpopulations in the entire enriched population, before and after migration (without SDF-1 [spontaneous] or toward a gradient of stromal cell–derived factor 1 [SDF-1]).
Enriched cells, which contain both CD34+CD38−/low and CD34−CD38−/lowLin−cells (Figure 2BIII), were assayed in a transwell migration assay.