VH segment usage in normal bone marrow and normal peripheral blood
| . | VH families . | Total VH . | |||||
|---|---|---|---|---|---|---|---|
| VH1/VH7 . | VH2 . | VH3 . | VH4 . | VH5 . | VH6 . | ||
| Normal BM (6 donors) | |||||||
| Total reflected density of PCR ladders | 575.3 | 210.2 | 2072.6 | 962.3 | 162.6 | 23.0 | 4005.9 |
| Mean reflected density per donor | 95.9 | 35.0 | 345.4 | 160.4 | 27.1 | 3.8 | 667.6 |
| Mean relative frequencies (%) | (14.4) | (5.2) | (51.7) | (24) | (4.1) | (0.6) | |
| SD (CV %) | 51 (53) | 24 (68) | 22 (6) | 29 (18) | 17 (61) | 3 (78) | |
| Total no. PCR-amplified bands | 90 | 60 | 126 | 116 | 60 | 20 | 472 |
| Normal PB (6 donors) | |||||||
| Total reflected density of PCR ladders | 980.8 | 550.7 | 3336.1 | 2501.0 | 644.0 | 390.8 | 8403.4 |
| Mean reflected density per donor | 163.5 | 91.8 | 556.0 | 416.8 | 107.3 | 65.1 | 1400.6 |
| Mean relative frequencies (%) | (11.7) | (6.6) | (39.7) | (29.8) | (7.7) | (4.7) | |
| SD (CV %) | 59 (36) | 38 (41) | 247 (44) | 197 (47) | 41 (38) | 48 (74) | |
| Total no. PCR-amplified bands | 85 | 60 | 137 | 129 | 69 | 45 | 525 |
| . | VH families . | Total VH . | |||||
|---|---|---|---|---|---|---|---|
| VH1/VH7 . | VH2 . | VH3 . | VH4 . | VH5 . | VH6 . | ||
| Normal BM (6 donors) | |||||||
| Total reflected density of PCR ladders | 575.3 | 210.2 | 2072.6 | 962.3 | 162.6 | 23.0 | 4005.9 |
| Mean reflected density per donor | 95.9 | 35.0 | 345.4 | 160.4 | 27.1 | 3.8 | 667.6 |
| Mean relative frequencies (%) | (14.4) | (5.2) | (51.7) | (24) | (4.1) | (0.6) | |
| SD (CV %) | 51 (53) | 24 (68) | 22 (6) | 29 (18) | 17 (61) | 3 (78) | |
| Total no. PCR-amplified bands | 90 | 60 | 126 | 116 | 60 | 20 | 472 |
| Normal PB (6 donors) | |||||||
| Total reflected density of PCR ladders | 980.8 | 550.7 | 3336.1 | 2501.0 | 644.0 | 390.8 | 8403.4 |
| Mean reflected density per donor | 163.5 | 91.8 | 556.0 | 416.8 | 107.3 | 65.1 | 1400.6 |
| Mean relative frequencies (%) | (11.7) | (6.6) | (39.7) | (29.8) | (7.7) | (4.7) | |
| SD (CV %) | 59 (36) | 38 (41) | 247 (44) | 197 (47) | 41 (38) | 48 (74) | |
| Total no. PCR-amplified bands | 85 | 60 | 137 | 129 | 69 | 45 | 525 |
BM indicates bone marrow; PCR, polymerase chain reaction; CV, coefficient of variation; PB, peripheral blood.