Checkboard analysis of monocyte migration across polycarbonate membrane after treatment with HFYLPM, MFYLPM, and HFYLPm
| Below (nM) . | Above (nM) . | |||
|---|---|---|---|---|
| 0 . | 10 . | 100 . | 1000 . | |
| HFYLPM | ||||
| 0 | 10.5 ± 3.42 | 11.0 ± 2.54 | 13.3 ± 2.40 | 10.0 ± 2.57 |
| 10 | 312 ± 23.0 | 47.7 ± 7.43 | 14.0 ± 3.97 | 10.7 ± 2.21 |
| 100 | 494 ± 34.0 | 366 ± 26.1 | 54.0 ± 14.5 | 19.3 ± 11.4 |
| 1000 | 612 ± 66.7 | 505 ± 53.7 | 210 ± 45.6 | 62.3 ± 21.0 |
| MFYLPM | ||||
| 0 | 16.3 ± 4.51 | 11.3 ± 2.42 | 15.0 ± 3.50 | 11.0 ± 2.67 |
| 10 | 198 ± 32.3 | 33.7 ± 8.93 | 16.0 ± 3.16 | 12.3 ± 3.43 |
| 100 | 557 ± 29.5 | 425 ± 48.3 | 66.3 ± 14.9 | 18.0 ± 4.05 |
| 1000 | 484 ± 43.6 | 395 ± 38.5 | 198 ± 26.7 | 76.7 ± 18.9 |
| HFYLPm | ||||
| 0 | 16.3 ± 3.22 | 11.3 ± 3.20 | 15.0 ± 4.82 | 11.0 ± 2.33 |
| 10 | 313 ± 32.4 | 53.7 ± 12.4 | 20.0 ± 5.56 | 12.3 ± 7.91 |
| 100 | 527 ± 29.5 | 325 ± 28.4 | 56.3 ± 9.37 | 28.0 ± 8.06 |
| 1000 | 425 ± 74.0 | 295 ± 56.6 | 98.3 ± 26.9 | 76.7 ± 17.8 |
| Below (nM) . | Above (nM) . | |||
|---|---|---|---|---|
| 0 . | 10 . | 100 . | 1000 . | |
| HFYLPM | ||||
| 0 | 10.5 ± 3.42 | 11.0 ± 2.54 | 13.3 ± 2.40 | 10.0 ± 2.57 |
| 10 | 312 ± 23.0 | 47.7 ± 7.43 | 14.0 ± 3.97 | 10.7 ± 2.21 |
| 100 | 494 ± 34.0 | 366 ± 26.1 | 54.0 ± 14.5 | 19.3 ± 11.4 |
| 1000 | 612 ± 66.7 | 505 ± 53.7 | 210 ± 45.6 | 62.3 ± 21.0 |
| MFYLPM | ||||
| 0 | 16.3 ± 4.51 | 11.3 ± 2.42 | 15.0 ± 3.50 | 11.0 ± 2.67 |
| 10 | 198 ± 32.3 | 33.7 ± 8.93 | 16.0 ± 3.16 | 12.3 ± 3.43 |
| 100 | 557 ± 29.5 | 425 ± 48.3 | 66.3 ± 14.9 | 18.0 ± 4.05 |
| 1000 | 484 ± 43.6 | 395 ± 38.5 | 198 ± 26.7 | 76.7 ± 18.9 |
| HFYLPm | ||||
| 0 | 16.3 ± 3.22 | 11.3 ± 3.20 | 15.0 ± 4.82 | 11.0 ± 2.33 |
| 10 | 313 ± 32.4 | 53.7 ± 12.4 | 20.0 ± 5.56 | 12.3 ± 7.91 |
| 100 | 527 ± 29.5 | 325 ± 28.4 | 56.3 ± 9.37 | 28.0 ± 8.06 |
| 1000 | 425 ± 74.0 | 295 ± 56.6 | 98.3 ± 26.9 | 76.7 ± 17.8 |
Monocyte migration was analyzed for 2 hours across a polycarbonate membrane with 5 μm pore size. Various concentrations of HFYLPM, MFYLPM, or HFYLPm were placed in the upper and lower compartments of the chambers. Data are presented as mean ± SE for migrated monocytes in 5 high-power fields counted in triplicate of 2 independent experiments.