Cytotoxicity of human telomerase reverse transcriptase peptide–specific cytotoxic T-lymphocyte clones to leukemia cell lines and normal cells
| Target cells . | HLA-A24 . | Telomerase activity* . | % Specific lysis† . | |||||
|---|---|---|---|---|---|---|---|---|
| KYO-1 . | HAS-1 . | |||||||
| 10:1 . | 5:1 . | 2.5:1 . | 10:1 . | 5:1 . | 2.5:1 . | |||
| Leukemia cell lines | ||||||||
| KH88 | + | 1.996 | 15.2 | 12.8 | 9.8 | 11.2 | 8.6 | 4.4 |
| MEG01 | + | 2.009 | 24.2 | 18.6 | 12.0 | 27.2 | 22.2 | 19.6 |
| OUN-1 | + | 1.894 | 19.7 | 17.9 | 14.1 | 13.6 | 11.2 | 8.5 |
| KT-1 | − | 1.522 | 0 | 0 | 0 | 0 | 0 | 0 |
| HL60 | − | 1.892 | 0 | 0 | 0 | 0 | 0 | 0 |
| K562 | − | 1.524 | 0 | 0 | 0 | 1.2 | 0.6 | 1.0 |
| Normal PBMCs | ||||||||
| Autologous (KYO) | + | 0.335 | 0 | 0 | 0 | ND | ND | ND |
| Autologous (HAS) | + | 0.271 | ND | ND | ND | 0 | 0 | 0 |
| Allogeneic 1 | + | 0.315 | 0 | 0 | 0 | 0 | 0 | 0 |
| Allogeneic 2 | − | 0.362 | 0 | 0 | 0 | 0 | 0 | 0 |
| Normal foreskin fibroblasts | ||||||||
| Allogeneic 1 | + | 0.024 | 0 | 0 | 0 | 0 | 0 | 0 |
| Allogeneic 2 | − | 0.023 | 0 | 0 | 0 | 0 | 0 | 0 |
| Target cells . | HLA-A24 . | Telomerase activity* . | % Specific lysis† . | |||||
|---|---|---|---|---|---|---|---|---|
| KYO-1 . | HAS-1 . | |||||||
| 10:1 . | 5:1 . | 2.5:1 . | 10:1 . | 5:1 . | 2.5:1 . | |||
| Leukemia cell lines | ||||||||
| KH88 | + | 1.996 | 15.2 | 12.8 | 9.8 | 11.2 | 8.6 | 4.4 |
| MEG01 | + | 2.009 | 24.2 | 18.6 | 12.0 | 27.2 | 22.2 | 19.6 |
| OUN-1 | + | 1.894 | 19.7 | 17.9 | 14.1 | 13.6 | 11.2 | 8.5 |
| KT-1 | − | 1.522 | 0 | 0 | 0 | 0 | 0 | 0 |
| HL60 | − | 1.892 | 0 | 0 | 0 | 0 | 0 | 0 |
| K562 | − | 1.524 | 0 | 0 | 0 | 1.2 | 0.6 | 1.0 |
| Normal PBMCs | ||||||||
| Autologous (KYO) | + | 0.335 | 0 | 0 | 0 | ND | ND | ND |
| Autologous (HAS) | + | 0.271 | ND | ND | ND | 0 | 0 | 0 |
| Allogeneic 1 | + | 0.315 | 0 | 0 | 0 | 0 | 0 | 0 |
| Allogeneic 2 | − | 0.362 | 0 | 0 | 0 | 0 | 0 | 0 |
| Normal foreskin fibroblasts | ||||||||
| Allogeneic 1 | + | 0.024 | 0 | 0 | 0 | 0 | 0 | 0 |
| Allogeneic 2 | − | 0.023 | 0 | 0 | 0 | 0 | 0 | 0 |
PBMC indicates peripheral blood mononuclear cell; ND, not determined.
Telomerase activity was measured by TRAP assay.
Cytotoxicity of KYO-1 and HAS-1 to various leukemia cell lines, normal PBMCs, and normal foreskin fibroblasts in the absence of the hTERT peptide was determined by 4-hour 51Cr release assays at effector:target ratios of 10:1, 5:1, and 2.5:1.