Table 3. Methylation status of 5 genes... | American Society of Hematology

Table 3.

Methylation status of 5 genes examined in 3 AML cell lines by RLGS, and expression by reverse transcription–polymerase chain reaction following treatment with 5-aza-2′-deoxycytidine

Clone	Gene	CpG Island	PB	HL-60			Kasumi-1			TI-1
Clone	Gene	CpG Island	PB	RLGS	No tx	AzaC	RLGS	No tx	AzaC	RLGS	No tx	Aza C
2C40	DRD4	5′	—	M	—	—	M	—	—	M	—	—
3B36	CYP1B1	mid	+	U	+	+	M	—	+	M	+	+
3C01	GαOlf	5′	+	M	—	—	M	+	+	M	—	+
3D41	EST		+	M	+	+	M	—	+	M	+	+
5E34	WIT1	5′	—	M	+	+	M	+	+	U	+	+

Clone	Gene	CpG Island	PB	HL-60			Kasumi-1			TI-1
Clone	Gene	CpG Island	PB	RLGS	No tx	AzaC	RLGS	No tx	AzaC	RLGS	No tx	Aza C
2C40	DRD4	5′	—	M	—	—	M	—	—	M	—	—
3B36	CYP1B1	mid	+	U	+	+	M	—	+	M	+	+
3C01	GαOlf	5′	+	M	—	—	M	+	+	M	—	+
3D41	EST		+	M	+	+	M	—	+	M	+	+
5E34	WIT1	5′	—	M	+	+	M	+	+	U	+	+

The location of the CpG island in the gene is indicated. The methylation status of the gene on the RLGS profile is indicated by “M” if methylated and “U” if unmethylated. Cells were cultured with (Aza C) and without (No tx) 5-aza-2′-deoxycytidine at 2.5 μM for 72 hours. The absence of a band in reverse transcription–polymerase chain reactions is indicated by a dash, and its presence by a plus. Shaded areas indicate that expression was restored with demethylation.

PB indicates normal donor peripheral blood.

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