Table 1. Humoral immune response after... | American Society of Hematology

Table 1.

Humoral immune response after CD40-CD40L blockade

Treatment	Anti-fVIII antibody titer, μg/mL
	End of treatment	Challenge with fVIII (days after treatment)
	End of treatment	50	100	150
Hamster IgG plus fVIII	1.3	66	Died	—
	14	110	Died	—
	15	Died	—	—
	50	232	661	474
	145	103	180	185
fVIII alone	3.6	77	104	165
	4.3	83	168	65
	12	134	123	300
	19	77	51	120
	53	166	175	250
Anti-CD40L + fVIII (nontolerant)	0.02	0.96	14.8	182
	0.03	0.2	4.2	180
	0.05	0.9	36	185
	0.1	0.36	1.2	20
	0.15	60	258	300
	0.15	53	617	550
	0.39	4.3	186	276
	0.65	19	165	116
	0.9	0.08	117	338
	1.5	5.8	25	150
Anti-CD40L + fVIII (tolerant)	0.02	0.01	0.01	0.05
	0.05	0.06	0.08	0.05
	0.05	0.01	0.05	0.02
	0.05	0.09	0.08	0.04
	0.06	0.04	0.05	0.05
	0.08	0.04	0.07	0.05
	0.09	0.02	0.06	0.04
	0.1	0.15	0.11	0.5
	0.1	0.04	0.48	0.85
	0.12	0.1	0.05	0.08
	0.15	0.05	0.18	0.22
	0.35	0.06	0.04	0.16

Treatment	Anti-fVIII antibody titer, μg/mL
	End of treatment	Challenge with fVIII (days after treatment)
	End of treatment	50	100	150
Hamster IgG plus fVIII	1.3	66	Died	—
	14	110	Died	—
	15	Died	—	—
	50	232	661	474
	145	103	180	185
fVIII alone	3.6	77	104	165
	4.3	83	168	65
	12	134	123	300
	19	77	51	120
	53	166	175	250
Anti-CD40L + fVIII (nontolerant)	0.02	0.96	14.8	182
	0.03	0.2	4.2	180
	0.05	0.9	36	185
	0.1	0.36	1.2	20
	0.15	60	258	300
	0.15	53	617	550
	0.39	4.3	186	276
	0.65	19	165	116
	0.9	0.08	117	338
	1.5	5.8	25	150
Anti-CD40L + fVIII (tolerant)	0.02	0.01	0.01	0.05
	0.05	0.06	0.08	0.05
	0.05	0.01	0.05	0.02
	0.05	0.09	0.08	0.04
	0.06	0.04	0.05	0.05
	0.08	0.04	0.07	0.05
	0.09	0.02	0.06	0.04
	0.1	0.15	0.11	0.5
	0.1	0.04	0.48	0.85
	0.12	0.1	0.05	0.08
	0.15	0.05	0.18	0.22
	0.35	0.06	0.04	0.16

Mice were injected with hamster IgG plus fVIII, fVIII alone, or with anti-CD40L Ab plus fVIII (treatment); they were further challenged with 0.2 μg fVIII 3 times after the treatment. Anti-fVIII Ab titers were determined by ELISA.

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