Table 2.

Engraftment of primary T-cell acute lymphoblastic leukemia in cord blood preconditioned nonobese diabetic/X-linked severe combined immunodeficient mice

PatientCord blood MNC
(preconditioning)		Injected T-ALLEngraftmentMetastasisOther tissues*
Human leukemia
cells of mouse
bone marrow cells (%)		Percent human
leukemia cells
in mouse spleen
10155 25 × 106 36 × 106 85 78 Yes 
26710 50 × 106 30 × 106 89 92 Yes 
26710 10 × 106 5.0 × 106 83 68 Yes 
26710 10 × 106 0.5 × 106 59 26 Yes 
26710 10 × 106 2.3 × 106 87 54 Yes 
26710 10 × 106 2.5 × 106 82 71 Yes 
27531 40 × 106 5.0 × 106 29 55 Yes 
27531 50 × 106 1.0 × 106 29 44 Yes 
30749 50 × 106 3.3 × 106 95 98 Yes 
30749 10 × 106 3.3 × 106 92 95 Yes 
30749 10 × 106 3.3 × 106 91 95 Yes 
30749 10 × 106 1.6 × 106 93 87 Yes 
30749 10 × 106 1.6 × 106 94 92 Yes 
30749 10 × 106 1.6 × 106 93 95 Yes 
08916 20 × 106 4.9 × 106 34 60 Yes 
08916 20 × 106 4.9 × 106 34 49 Yes 
08948 20 × 106 5.1 × 106 38 57 Yes 
33205 25 × 106 5.0 × 106 85 86 Yes 
32564 25 × 106 6.0 × 106 77 87 Yes 
PatientCord blood MNC
(preconditioning)		Injected T-ALLEngraftmentMetastasisOther tissues*
Human leukemia
cells of mouse
bone marrow cells (%)		Percent human
leukemia cells
in mouse spleen
10155 25 × 106 36 × 106 85 78 Yes 
26710 50 × 106 30 × 106 89 92 Yes 
26710 10 × 106 5.0 × 106 83 68 Yes 
26710 10 × 106 0.5 × 106 59 26 Yes 
26710 10 × 106 2.3 × 106 87 54 Yes 
26710 10 × 106 2.5 × 106 82 71 Yes 
27531 40 × 106 5.0 × 106 29 55 Yes 
27531 50 × 106 1.0 × 106 29 44 Yes 
30749 50 × 106 3.3 × 106 95 98 Yes 
30749 10 × 106 3.3 × 106 92 95 Yes 
30749 10 × 106 3.3 × 106 91 95 Yes 
30749 10 × 106 1.6 × 106 93 87 Yes 
30749 10 × 106 1.6 × 106 94 92 Yes 
30749 10 × 106 1.6 × 106 93 95 Yes 
08916 20 × 106 4.9 × 106 34 60 Yes 
08916 20 × 106 4.9 × 106 34 49 Yes 
08948 20 × 106 5.1 × 106 38 57 Yes 
33205 25 × 106 5.0 × 106 85 86 Yes 
32564 25 × 106 6.0 × 106 77 87 Yes 
*

Metastasis to other tissues determined by hematoxylin-eosin staining of paraffin-embedded sections of liver, kidney, lung, and so on, as described in “Materials and methods.” See also Figure 4.

MNC indicates mononuclear cell; T-ALL, T-cell acute lymphoblastic leukemia.

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