Table 4. Characteristic changes in the... | American Society of Hematology

Table 4.

Characteristic changes in the 5′-noncoding regulatory region of the BCL-6 gene in Hodgkin disease cell lines

Cell line	Genotype	Nucleotide changes
L540^4-150	T	None
Holden	T	None
KMH2^4-150	B	(a) 607G>A
L428^4-150	B	(a) 201T>G, 246T>C
		(b) 82G>A, 92T>A, 119C>T, 290T>C, 301A>G, 337A>C, 445A>T, 484T>C
L591	B	(a) 122G>C^4-151
		(b) None
L1236^‡	B	(a) 123C>G^4-151, 220G>C, 299G>A, 390A>C, 435C>T, 437G>A^4-153, 506delA, 580C>G
		(b) 181A>G, 235T>C, 279delT, 314A>T, 327A>G, 328G>T, 377G>C, 427T>A, 445A>C, 498G>C, 510C>T, 518G>A, 541T>A, 543C>G, 569T>G
		(c) 181A>G, 220G>C, 299G>A, 390A>C, 435C>T, 437G>A^4-153, 506delA, 580C>G

Cell line	Genotype	Nucleotide changes
L540^4-150	T	None
Holden	T	None
KMH2^4-150	B	(a) 607G>A
L428^4-150	B	(a) 201T>G, 246T>C
		(b) 82G>A, 92T>A, 119C>T, 290T>C, 301A>G, 337A>C, 445A>T, 484T>C
L591	B	(a) 122G>C^4-151
		(b) None
L1236^‡	B	(a) 123C>G^4-151, 220G>C, 299G>A, 390A>C, 435C>T, 437G>A^4-153, 506delA, 580C>G
		(b) 181A>G, 235T>C, 279delT, 314A>T, 327A>G, 328G>T, 377G>C, 427T>A, 445A>C, 498G>C, 510C>T, 518G>A, 541T>A, 543C>G, 569T>G
		(c) 181A>G, 220G>C, 299G>A, 390A>C, 435C>T, 437G>A^4-153, 506delA, 580C>G

Reference sequence corresponds to AF191831.5 If different alleles were detected, they are marked with (a), (b), or (c). Mutations reported as recurrent in FL and DLBCL5 are in bold.

F4-150

The same results have already been described in Carbone et al.13 In addition, mutation 445A>T was found in cell line L428. Furthermore, we were able to discriminate both alleles in this cell line.

F4-151

Mutation is located at an RGWY motif.5

‡

The presence of 3 alleles in cell line L1236 is in harmony with its karyotype.22

F4-153

Site of mutation also described in reactive B cells.5

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