Table 1. Results of replating... | American Society of Hematology

Table 1.

Results of replating granulocyte-macrophage colony-forming unit colonies with p16^INK4a expression abrogated in human cells or deleted in murine cells

	No.experiments	Replating colonies, %	Secondary colonies^*	AUC
Human cells
Control human marrow	3	20 ± 4	53 ± 26	16 ± 4
CDK4-transduced human marrow	3	48 ± 5^†	433 ± 260^†	83 ± 25^†
Murine cells
Wild-type mouse	3	34 ± 8	112 ± 34	37 ± 6
p16^INK4a−/−mouse	3	54 ± 6^†	608 ± 157^†	128 ± 17^†
p16^INK4a−/− cell lines	15	97 ± 5^†	2 404 ± 402^†	343 ± 149^†

	No.experiments	Replating colonies, %	Secondary colonies^*	AUC
Human cells
Control human marrow	3	20 ± 4	53 ± 26	16 ± 4
CDK4-transduced human marrow	3	48 ± 5^†	433 ± 260^†	83 ± 25^†
Murine cells
Wild-type mouse	3	34 ± 8	112 ± 34	37 ± 6
p16^INK4a−/−mouse	3	54 ± 6^†	608 ± 157^†	128 ± 17^†
p16^INK4a−/− cell lines	15	97 ± 5^†	2 404 ± 402^†	343 ± 149^†

Data are presented as mean ± SD.

AUC indicates area under the curve; CDK4 indicates cyclin-dependent kinase 4.

There were 120 primary colonies.

†

P < .05 compared with controls (Mann-Whitney test).

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