CCP 25 SSOPs and hybridization conditions
| Probe . | Sequence . | Stringency temperature . | Wash condition salt . |
|---|---|---|---|
| 4795A | 5′-CTA CTA ATA AAT GCA C-3′ | 37°C | 0.8x |
| 4795G | 5′-CTA CTA ATG AAT GCA C-3′ | 40°C | 0.8x |
| 4828A | 5′-TGC AAT TAG AGT ACC-3′ | 39°C | 1.0x |
| 4828C | 5′-GGT ACT CCA ATT GCA-3′ | 44°C | 0.4x |
| 4855T | 5′-TTT CTT TTC CCT CAC-3′ | 37°C | 1.0x |
| 4855A | 5′-TTT CTT TAC CCT CAC-3′ | 37°C | 0.8x |
| 4803 | 5′-AGC TCC AGA AGT TGA-3′ | 44°C | 1.0x |
| Probe . | Sequence . | Stringency temperature . | Wash condition salt . |
|---|---|---|---|
| 4795A | 5′-CTA CTA ATA AAT GCA C-3′ | 37°C | 0.8x |
| 4795G | 5′-CTA CTA ATG AAT GCA C-3′ | 40°C | 0.8x |
| 4828A | 5′-TGC AAT TAG AGT ACC-3′ | 39°C | 1.0x |
| 4828C | 5′-GGT ACT CCA ATT GCA-3′ | 44°C | 0.4x |
| 4855T | 5′-TTT CTT TTC CCT CAC-3′ | 37°C | 1.0x |
| 4855A | 5′-TTT CTT TAC CCT CAC-3′ | 37°C | 0.8x |
| 4803 | 5′-AGC TCC AGA AGT TGA-3′ | 44°C | 1.0x |
SSOP indicates sequence-specific oligonucleotide probe. Underlined base pair indicates the polymorphic site.