Polymerase chain reaction primers and complement receptor 1 amplification strategies
| Exon . | CCP . | Primer . | LHR . | Sequence . | Annealing temperature . | Amplicon length (bp) . |
|---|---|---|---|---|---|---|
| 22/23 | 19/20a | 19/20aUP | C | 5′-ACC TCT GAC TAG CTA TGA GGT CTT C-3′ | 65°C | 518 |
| 19/20aDN | 5′-TTA AGC TCA AGA GTC ACT CTG CAG-3′ | |||||
| 24 | 20b | 20bUP | C | 5′-AAT GGT GCA TTC ATC CAG CCA CTA-3′ | 65°C | 164 |
| 20bDN | 5′-AAA CAG AAA GAT CCA GAC TGA ACA AGT-3′ | |||||
| 25 | 21 | 21UP | C | 5′-AGT ATG ACA ACT GTA GAA TCA CCT TG-3′ | 65°C | 336 |
| 21DN | 5′-CCA TTT AGG ATA TGT GGA AGT GGA TA-3′ | |||||
| 26 | 22 | 22UP | D | 5′-GAG GTA GGG TGG AAG TCT CTC TG-3′ | 65°C | 279 |
| 22DN | 5′-GCT TTA ACA GAC ATG AAG TAG TTC C-3′ | |||||
| 27 | 23a | 23aUP | D | 5′-ACT CTA TAC TTA ATC CCA AAT TCT GC-3′ | 65°C | 184 |
| 23aDN | 5′-TCT GCT GTT CTG GAA CTC ATA TTC-3′ | |||||
| 28 | 23b | 23bUP | D | 5′-ATG GTG AGT TAA ATG GGA AAT ATG-3′ | 60°C | 200 |
| 23bDN | 5′-AGG GGG CCA GGC AGG ATC ACA C-3′ | |||||
| 29 | 24/25 | 24/25UP | D | 5′-TAA AAA ATA AGC TGT TTT ACC ATA CTC-3′ | 60°C | 476 |
| 24/25DN | 5′-CCC TCA CAC CCA GCA AAG TC-3′ | |||||
| 30/31 | 26/27a | 26/27aUP | D | 5′-TTT TAA GCC ATC TGG TAA GCA TAA G-3′ | 65°C | 505 |
| 26/27aDN | 5′-GAT CTC AAG AGA GTG ATT CTC CAC AC-3′ | |||||
| 32 | 27b | 27bUP | D | 5′-AGA TGG GAA TTG CTC ACA CAT TTG G-3′ | 58°C | 185 |
| 27bDN | 5′-CTG GAA AAC GAA AAT AAG GAG AGC-3′ | |||||
| 33 | 28 | 28UP | D | 5′-GTC ACA GGT CAC TAT TGT TTC AG-3′ | 65°C | 332 |
| 28DN | 5′-CCA TTT AGG ATA TGT GGA AGC AGG-3′ | |||||
| 34 | 29 | 29UP | 5′-TGT GTT TGT GTG GGA ACT TGT TCT TAG-3′ | 65°C | 247 | |
| 29DN | 5′-CCA TAC AAC CCA GCA AGA ATA AGG-3′ | |||||
| 35 | 30 | 30UP | 5′-TAA TTT CAG AAG TAA ATT GTA GCT TCC-3′ | 63°C | 233 | |
| 30DN | 5′-CAT GAC CGG AAG ATC CAC ATT C-3′ | |||||
| 36 | Tma | TmaUP | 5′-ATG CCA GAG TGA TGT TTT GTG AC-3′ | 58°C | 86 | |
| TmaDN | 5′-ATC AAA ACT TTC ATA AAA CTT ACC-3′ | |||||
| 37 | Tmb | TmbUP | 5′-CTT GTA AGT TAT ATT TTC CAT GC-3′ | 58°C | 200 | |
| TmbDN | 5′-TGT CTT TTA CTT TCT TTT GAC TTC C-3′ | |||||
| 38 | Cyto | CytoUP | 5′-GAC AGT TTT TCT ATT TTT TTC TCT GC-3′ | 65°C | 180 | |
| CytoDN | 5′-GAG TTG TTG AAA ACA TTT TGA ATT CAG-3′ |
| Exon . | CCP . | Primer . | LHR . | Sequence . | Annealing temperature . | Amplicon length (bp) . |
|---|---|---|---|---|---|---|
| 22/23 | 19/20a | 19/20aUP | C | 5′-ACC TCT GAC TAG CTA TGA GGT CTT C-3′ | 65°C | 518 |
| 19/20aDN | 5′-TTA AGC TCA AGA GTC ACT CTG CAG-3′ | |||||
| 24 | 20b | 20bUP | C | 5′-AAT GGT GCA TTC ATC CAG CCA CTA-3′ | 65°C | 164 |
| 20bDN | 5′-AAA CAG AAA GAT CCA GAC TGA ACA AGT-3′ | |||||
| 25 | 21 | 21UP | C | 5′-AGT ATG ACA ACT GTA GAA TCA CCT TG-3′ | 65°C | 336 |
| 21DN | 5′-CCA TTT AGG ATA TGT GGA AGT GGA TA-3′ | |||||
| 26 | 22 | 22UP | D | 5′-GAG GTA GGG TGG AAG TCT CTC TG-3′ | 65°C | 279 |
| 22DN | 5′-GCT TTA ACA GAC ATG AAG TAG TTC C-3′ | |||||
| 27 | 23a | 23aUP | D | 5′-ACT CTA TAC TTA ATC CCA AAT TCT GC-3′ | 65°C | 184 |
| 23aDN | 5′-TCT GCT GTT CTG GAA CTC ATA TTC-3′ | |||||
| 28 | 23b | 23bUP | D | 5′-ATG GTG AGT TAA ATG GGA AAT ATG-3′ | 60°C | 200 |
| 23bDN | 5′-AGG GGG CCA GGC AGG ATC ACA C-3′ | |||||
| 29 | 24/25 | 24/25UP | D | 5′-TAA AAA ATA AGC TGT TTT ACC ATA CTC-3′ | 60°C | 476 |
| 24/25DN | 5′-CCC TCA CAC CCA GCA AAG TC-3′ | |||||
| 30/31 | 26/27a | 26/27aUP | D | 5′-TTT TAA GCC ATC TGG TAA GCA TAA G-3′ | 65°C | 505 |
| 26/27aDN | 5′-GAT CTC AAG AGA GTG ATT CTC CAC AC-3′ | |||||
| 32 | 27b | 27bUP | D | 5′-AGA TGG GAA TTG CTC ACA CAT TTG G-3′ | 58°C | 185 |
| 27bDN | 5′-CTG GAA AAC GAA AAT AAG GAG AGC-3′ | |||||
| 33 | 28 | 28UP | D | 5′-GTC ACA GGT CAC TAT TGT TTC AG-3′ | 65°C | 332 |
| 28DN | 5′-CCA TTT AGG ATA TGT GGA AGC AGG-3′ | |||||
| 34 | 29 | 29UP | 5′-TGT GTT TGT GTG GGA ACT TGT TCT TAG-3′ | 65°C | 247 | |
| 29DN | 5′-CCA TAC AAC CCA GCA AGA ATA AGG-3′ | |||||
| 35 | 30 | 30UP | 5′-TAA TTT CAG AAG TAA ATT GTA GCT TCC-3′ | 63°C | 233 | |
| 30DN | 5′-CAT GAC CGG AAG ATC CAC ATT C-3′ | |||||
| 36 | Tma | TmaUP | 5′-ATG CCA GAG TGA TGT TTT GTG AC-3′ | 58°C | 86 | |
| TmaDN | 5′-ATC AAA ACT TTC ATA AAA CTT ACC-3′ | |||||
| 37 | Tmb | TmbUP | 5′-CTT GTA AGT TAT ATT TTC CAT GC-3′ | 58°C | 200 | |
| TmbDN | 5′-TGT CTT TTA CTT TCT TTT GAC TTC C-3′ | |||||
| 38 | Cyto | CytoUP | 5′-GAC AGT TTT TCT ATT TTT TTC TCT GC-3′ | 65°C | 180 | |
| CytoDN | 5′-GAG TTG TTG AAA ACA TTT TGA ATT CAG-3′ |
CCP indicates complement control protein repeat; LHR, long homologous repeat (Figure 1); UP, sense; DN, antisense; Tm, transmembrane; Cyto, cytoplasmic.