Primers, conditions, and products of cytokine and cytokine receptor RT-PCR
| Target cDNA . | Forward primer . | Reverse primer . | T (°C) . | Product (bp) . |
|---|---|---|---|---|
| IL-4 | GTTCTTCCTGCTAGCATGTGC | CATGATCGTCTTTAGCCTTTCC | 57 | 400 |
| IL-10 | ATCTCCGAGATGCCTTCAGCAG | GCATTCTTCACCTGCTCCACG | 56 | 307 |
| IL-12p40 | AAGATGTGTCACCAGCAGTTGG | CGCAGAATGTCAGGGAGAAGT | 57 | 825 |
| IL-12Rβ2 | TTGGAGTGAATCATTGAGAGCAC | TTCTCTGAAATCAGAGCAGACAC | 56 | 546 |
| β-actin | GTGATGGTGGGCATGGGTC | CCGTGGCCATCTCTTGCTC | 58 | 561 |
| Target cDNA . | Forward primer . | Reverse primer . | T (°C) . | Product (bp) . |
|---|---|---|---|---|
| IL-4 | GTTCTTCCTGCTAGCATGTGC | CATGATCGTCTTTAGCCTTTCC | 57 | 400 |
| IL-10 | ATCTCCGAGATGCCTTCAGCAG | GCATTCTTCACCTGCTCCACG | 56 | 307 |
| IL-12p40 | AAGATGTGTCACCAGCAGTTGG | CGCAGAATGTCAGGGAGAAGT | 57 | 825 |
| IL-12Rβ2 | TTGGAGTGAATCATTGAGAGCAC | TTCTCTGAAATCAGAGCAGACAC | 56 | 546 |
| β-actin | GTGATGGTGGGCATGGGTC | CCGTGGCCATCTCTTGCTC | 58 | 561 |
RT-PCR indicates reverse transcriptase–polymerase chain reaction; cDNA, complementary DNA; T, annealing temperature; bp, base pairs; IL, interleukin; and R, receptor.