Characteristics of 36 acute myeloid leukemia patients, by number of genes that are polymerase chain reaction-positive for methylation
| . | 0-2 PCR+ genes (N = 16) . | 3-7 PCR+ genes (N = 13) . | 8-13 PCR+ genes (N = 7) . | All patients (N = 36) . | ||||
|---|---|---|---|---|---|---|---|---|
| No. . | % . | No. . | % . | No. . | % . | No. . | % . | |
| Gender | ||||||||
| Female | 8 | 50% | 6 | 46% | 3 | 43% | 17 | 47% |
| FAB | ||||||||
| M1 | 4 | 25% | 2 | 15% | 3 | 43% | 9 | 25% |
| M2 | 5 | 31% | 7 | 54% | 3 | 43% | 15 | 42% |
| M3 | 2 | 13% | 1 | 8% | 0 | 4 | 11% | |
| M4, M5 | 5 | 32% | 2 | 16% | 0 | 6 | 17% | |
| M0/AML, not otherwise specified | 0 | 1 | 8% | 1 | 14% | 2 | 6% | |
| MDR1, based on MRK164-150,4-151 | ||||||||
| Negative | 7 | 64% | 6 | 55% | 7 | 100% | 20 | 69% |
| Dim | 1 | 9% | 3 | 27% | 0 | 4 | 14% | |
| Moderate/bright | 3 | 27% | 2 | 18% | 0 | 5 | 17% | |
| Unknown | 5 | — | 2 | — | 0 | — | 7 | — |
| CsA-inhibited DiOC2efflux4-150,‡ | ||||||||
| Negative | 7 | 58% | 7 | 58% | 7 | 100% | 21 | 68% |
| Dim | 1 | 8% | 0 | 0 | 1 | 3% | ||
| Moderate/bright | 4 | 33% | 5 | 42% | 0 | 9 | 29% | |
| Unknown | 4 | — | 1 | — | 0 | — | 5 | — |
| Age, y, median (range) | 55 (29-69) | NA | 35 (18-63) | NA | 27 (19-43) | NA | 39 | NA |
| Marrow blasts, %, median (range) | 83 (25-98) | NA | 81 (13-97) | NA | 95 (8-97) | NA | 87 | NA |
| WBC, 1 × 109/L (1000 μL), median (range) | 54.3 (1-270) | NA | 38.3 (6-308) | NA | 19.7 (2-103) | NA | 37.7 | NA |
| Hemoglobin, g/dL, | 9.3 (7-14) | NA | 10.0 (6-12) | NA | 8.2 (6-15) | NA | 9.1 | NA |
| Platelets, 1000 μL, median (range) | 41 (3-120) | NA | 43 (10-141) | NA | 56 (24-167) | NA | 43 | NA |
| . | 0-2 PCR+ genes (N = 16) . | 3-7 PCR+ genes (N = 13) . | 8-13 PCR+ genes (N = 7) . | All patients (N = 36) . | ||||
|---|---|---|---|---|---|---|---|---|
| No. . | % . | No. . | % . | No. . | % . | No. . | % . | |
| Gender | ||||||||
| Female | 8 | 50% | 6 | 46% | 3 | 43% | 17 | 47% |
| FAB | ||||||||
| M1 | 4 | 25% | 2 | 15% | 3 | 43% | 9 | 25% |
| M2 | 5 | 31% | 7 | 54% | 3 | 43% | 15 | 42% |
| M3 | 2 | 13% | 1 | 8% | 0 | 4 | 11% | |
| M4, M5 | 5 | 32% | 2 | 16% | 0 | 6 | 17% | |
| M0/AML, not otherwise specified | 0 | 1 | 8% | 1 | 14% | 2 | 6% | |
| MDR1, based on MRK164-150,4-151 | ||||||||
| Negative | 7 | 64% | 6 | 55% | 7 | 100% | 20 | 69% |
| Dim | 1 | 9% | 3 | 27% | 0 | 4 | 14% | |
| Moderate/bright | 3 | 27% | 2 | 18% | 0 | 5 | 17% | |
| Unknown | 5 | — | 2 | — | 0 | — | 7 | — |
| CsA-inhibited DiOC2efflux4-150,‡ | ||||||||
| Negative | 7 | 58% | 7 | 58% | 7 | 100% | 21 | 68% |
| Dim | 1 | 8% | 0 | 0 | 1 | 3% | ||
| Moderate/bright | 4 | 33% | 5 | 42% | 0 | 9 | 29% | |
| Unknown | 4 | — | 1 | — | 0 | — | 5 | — |
| Age, y, median (range) | 55 (29-69) | NA | 35 (18-63) | NA | 27 (19-43) | NA | 39 | NA |
| Marrow blasts, %, median (range) | 83 (25-98) | NA | 81 (13-97) | NA | 95 (8-97) | NA | 87 | NA |
| WBC, 1 × 109/L (1000 μL), median (range) | 54.3 (1-270) | NA | 38.3 (6-308) | NA | 19.7 (2-103) | NA | 37.7 | NA |
| Hemoglobin, g/dL, | 9.3 (7-14) | NA | 10.0 (6-12) | NA | 8.2 (6-15) | NA | 9.1 | NA |
| Platelets, 1000 μL, median (range) | 41 (3-120) | NA | 43 (10-141) | NA | 56 (24-167) | NA | 43 | NA |
PCR indicates polymerase chain reaction; FAB, French-American-British; CsA, ; DiOC2WBC, white blood count.
Categories based on Kolmogorov-Smirnov D-value as defined in Leith et al.36
MRK16: negative D < 0.15, dim 0.15 ≤ D < 0.20, moderate 0.20 ≤ D < 0.30, bright D ≥ 0.30.
Efflux: negative D < 0.20, dim 0.20 ≤ D < 0.25, moderate 0.25 ≤ D < 0.40, bright D ≥ 0.40.