Poly(l-lysine) molecular weight and DNA type affect the body distribution of pLL/DNA complexes in mice
| . | Carcass . | Liver . | Lungs . | Kidneys . | Intestine . | Spleen . |
|---|---|---|---|---|---|---|
| pLL20/DNAct | 10.8 ± 3.6 | 78.9 ± 7.3 | 1.7 ± 0.5 | 0.8 ± 0.1 | 1.7 ± 0.7 | 9.1 ± 4.2 |
| pLL20/DNAcp | 24.5 ± 5.6 | 60.3 ± 8.5 | 2.0 ± 0.4 | 1.9 ± 0.6 | 4.2 ± 1.1 | 6.9 ± 1.2 |
| pLL20/DNAlp | 16.3 ± 2.3 | 71.4 ± 5.2 | 1.0 ± 0.2 | 1.8 ± 0.3 | 4.6 ± 2.3 | 5.0 ± 1.8 |
| pLL211/DNAct | 36.6 ± 6.8 | 42.8 ± 8.8 | 2.8 ± 0.1 | 1.9 ± 1.0 | 4.7 ± 1.2 | 8.8 ± 2.9 |
| pLL211/DNAcp | 33.4 ± 3.2 | 46.9 ± 3.6 | 2.9 ± 0.4 | 1.8 ± 0.4 | 3.6 ± 0.3 | 12.5 ± 1.6 |
| pLL211/DNAlp | 30.9 ± 0.8 | 50.6 ± 2.9 | 2.5 ± 0.8 | 1.6 ± 0.4 | 2.5 ± 0.3 | 12.1 ± 0.8 |
| DNA* | 9.4 ± 2.9 | 84.9 ± 2.0 | 1.1 ± 0.1 | 6.5 ± 1.8 | 5.1 ± 1.3 | 0.4 ± 0.1 |
| . | Carcass . | Liver . | Lungs . | Kidneys . | Intestine . | Spleen . |
|---|---|---|---|---|---|---|
| pLL20/DNAct | 10.8 ± 3.6 | 78.9 ± 7.3 | 1.7 ± 0.5 | 0.8 ± 0.1 | 1.7 ± 0.7 | 9.1 ± 4.2 |
| pLL20/DNAcp | 24.5 ± 5.6 | 60.3 ± 8.5 | 2.0 ± 0.4 | 1.9 ± 0.6 | 4.2 ± 1.1 | 6.9 ± 1.2 |
| pLL20/DNAlp | 16.3 ± 2.3 | 71.4 ± 5.2 | 1.0 ± 0.2 | 1.8 ± 0.3 | 4.6 ± 2.3 | 5.0 ± 1.8 |
| pLL211/DNAct | 36.6 ± 6.8 | 42.8 ± 8.8 | 2.8 ± 0.1 | 1.9 ± 1.0 | 4.7 ± 1.2 | 8.8 ± 2.9 |
| pLL211/DNAcp | 33.4 ± 3.2 | 46.9 ± 3.6 | 2.9 ± 0.4 | 1.8 ± 0.4 | 3.6 ± 0.3 | 12.5 ± 1.6 |
| pLL211/DNAlp | 30.9 ± 0.8 | 50.6 ± 2.9 | 2.5 ± 0.8 | 1.6 ± 0.4 | 2.5 ± 0.3 | 12.1 ± 0.8 |
| DNA* | 9.4 ± 2.9 | 84.9 ± 2.0 | 1.1 ± 0.1 | 6.5 ± 1.8 | 5.1 ± 1.3 | 0.4 ± 0.1 |
pLL/DNA complexes, formed with pLL20 or pLL211, and either DNAct, DNAcp, or DNAlp were administered into female Balb/c mice (0.1 mL of 10 μg/mL DNA) through tail vein injection. Mice were killed after 30 minutes, and the organs and carcasses were dissolved in 10 M NaOH at 75°C for 1 hour. Radioactivity in the samples was measured in a liquid scintillation analyzer (1900TR; Packard). The values are corrected for the amount of blood present in organs of decapitated mice.36
pLL indicates poly(l-lysine); DNAct, calf thymus DNA; DNAcp, closed circular plasmid DNA; DNAlp, linear plasmid DNA.
DNA value is that obtained after the administration of DNAct and the radioactive probe because there was no significant difference using either DNAcp or DNAlp. Results show the mean ± SD of 3 independent experiments.