Predicted amino acid sequences of the CDR3 loops of the BV2+ CD8+ peripheral blood mononuclear cells s of patient T19
| Sample . | BV . | BJ . | CDR3 . | Frequency . | Length (aa) . |
|---|---|---|---|---|---|
| T19/M0 | 2 | 2S1 | VLRGGGEQ | 1/18 | 8 |
| T19/M0 | 2 | 2S2 | EGGSGNTI | 1/18 | 8 |
| T19/M0 | 2 | 1S1 | VRDRGNTEA | 1/18 | 9 |
| T19/M0 | 2 | 2S1 | RRASSYNEQ | 1/18 | 9 |
| T19/M0 | 2 | 2S1 | IPRFADYEQ | 1/18 | 9 |
| T19/M0 | 2 | 2S2 | LEGTNTGEL | 1/18 | 9 |
| T19/M0 | 2 | 1S2 | PRQVLNYGY | 1/18 | 9 |
| T19/M0 | 2 | 1S1 | RDLKGLNTEA | 4/18 | 10 |
| T19/M0 | 2 | 2S1 | PSFTGRRDGY | 1/18 | 10 |
| T19/M0 | 2 | 2S5 | GAGGPHGETQ | 1/18 | 10 |
| T19/M0 | 2 | 2S5 | GRGGRNGRAQ | 1/18 | 10 |
| T19/M0 | 2 | 1S1 | RDSDRDNTEA | 2/18 | 10 |
| T19/M0 | 2 | 1S1 | NPGDSGYSPLH | 2/18 | 11 |
| T19/M12 | 2 | 1S2 | RTGGYGY | 1/9 | 7 |
| T19/M12 | 2 | 1S2 | SVPGNGY | 1/9 | 7 |
| T19/M12 | 2 | 1S1 | RDWDSEEA | 6/9 | 8 |
| T19/M12 | 2 | 1S1 | RDLKGLNTEA | 1/9 | 10 |
| T19/M20 | 2 | 1S1 | RGGIRA | 1/21 | 6 |
| T19/M20 | 2 | 1S1 | KQGNTEA | 1/21 | 7 |
| T19/M20 | 2 | 1S1 | RDWDSEEA | 11/21 | 8 |
| T19/M20 | 2 | 2S3 | RDLGQASW | 1/21 | 8 |
| T19/M20 | 2 | 1S2 | RKTGGAQN | 1/21 | 8 |
| T19/M20 | 2 | 2S6 | RDTDRANVL | 1/21 | 9 |
| T19/M20 | 2 | 1S5 | RDRQSNQPQ | 1/21 | 9 |
| T19/M20 | 2 | 1S1 | RDPPTASWYN | 1/21 | 10 |
| T19/M20 | 2 | 1S1 | TYRAISNQPQ | 1/21 | 10 |
| T19/M20 | 2 | 2S7 | RDGGFSTDTQ | 1/21 | 10 |
| T19/M20 | 2 | 1S1 | RETGGWGYTEA | 1/21 | 11 |
| T19/M0 | 7 | 2S3 | QDSGSEQ | 2/5 | 7 |
| T19/M0 | 7 | 1S1 | QAGGMRTEA | 3/5 | 9 |
| T19/M1 | 7 | 1S5 | LAAVSYEQ | 1/6 | 8 |
| T19/M1 | 7 | 2S5 | QGRSSYNEQ | 4/6 | 9 |
| T19/M1 | 7 | 1S1 | QAGGMRTEA | 1/6 | 9 |
| T19/M3 | 7 | 2S3 | QVQETQ | 1/8 | 6 |
| T19/M3 | 7 | 1S2 | HLTGAGGY | 1/8 | 8 |
| T19/M3 | 7 | 2S5 | QGRSSYNEQ | 6/8 | 9 |
| T19/M6 | 7 | 2S3 | QGTAINEQ | 1/9 | 8 |
| T19/M6 | 7 | 2S5 | QGRSSYNEQ | 6/9 | 9 |
| T19/M6 | 7 | 2S3 | QLYQFTYEQ | 1/9 | 9 |
| T19/M6 | 7 | 1S5 | QDGTGRRTPQ | 1/9 | 10 |
| T19/M12 | 7 | 2S5 | QGRSSYNEQ | 10/10 | 9 |
| T19/M20 | 7 | 2S5 | QGRSSYNEQ | 5/5 | 9 |
| T19/M0 | 8 | 1S1 | LWNTEA | 1/20 | 6 |
| T19/M0 | 8 | 1S1 | AGPGTEA | 1/20 | 7 |
| T19/M0 | 8 | 2S1 | LPGGSEQ | 1/20 | 7 |
| T19/M0 | 8 | 2S3 | LFWEYEQ | 1/20 | 7 |
| T19/M0 | 8 | 2S3 | LEEVYEQ | 1/20 | 7 |
| T19/M0 | 8 | 2S3 | SGPIYEQ | 1/20 | 7 |
| T19/M0 | 8 | 2S3 | LASGQETQ | 1/20 | 8 |
| T19/M0 | 8 | 2S1 | LTSAYNEQ | 1/20 | 8 |
| T19/M0 | 8 | 2S3 | FGGGADTQ | 1/20 | 8 |
| T19/M0 | 8 | 2S1 | GLLVLGDEQ | 5/20 | 9 |
| T19/M0 | 8 | 2S3 | LGQGTTYEQ | 1/20 | 9 |
| T19/M0 | 8 | 1S3 | RPRGSGNTI | 1/20 | 9 |
| T19/M0 | 8 | 2S3 | PSRDDSNTGEL | 1/20 | 11 |
| T19/M0 | 8 | 1S1 | LEDRDPRNTEA | 2/20 | 11 |
| T19/M0 | 8 | 1S2 | LLIGGQPFNYGY | 1/20 | 12 |
| T19/M20 | 8 | 2S3 | LFWEYEQ | 2/3 | 7 |
| T19/M20 | 8 | 2S1 | TQAGGDTQ | 1/13 | 8 |
| T19/M20 | 8 | 2S1 | LTTSAYEQ | 1/13 | 8 |
| T19/M20 | 8 | 2S1 | LGRGGNEQ | 1/13 | 8 |
| T19/M20 | 8 | 2S1 | GLLVLGDEQ | 4/13 | 9 |
| T19/M20 | 8 | 2S5 | LGQGTTYEQ | 1/13 | 9 |
| T19/M20 | 8 | 1S1 | LEDRDPRNTEA | 2/13 | 11 |
| T19/M20 | 8 | 1S2 | LLIGGQPFNYGY | 1/13 | 12 |
| Sample . | BV . | BJ . | CDR3 . | Frequency . | Length (aa) . |
|---|---|---|---|---|---|
| T19/M0 | 2 | 2S1 | VLRGGGEQ | 1/18 | 8 |
| T19/M0 | 2 | 2S2 | EGGSGNTI | 1/18 | 8 |
| T19/M0 | 2 | 1S1 | VRDRGNTEA | 1/18 | 9 |
| T19/M0 | 2 | 2S1 | RRASSYNEQ | 1/18 | 9 |
| T19/M0 | 2 | 2S1 | IPRFADYEQ | 1/18 | 9 |
| T19/M0 | 2 | 2S2 | LEGTNTGEL | 1/18 | 9 |
| T19/M0 | 2 | 1S2 | PRQVLNYGY | 1/18 | 9 |
| T19/M0 | 2 | 1S1 | RDLKGLNTEA | 4/18 | 10 |
| T19/M0 | 2 | 2S1 | PSFTGRRDGY | 1/18 | 10 |
| T19/M0 | 2 | 2S5 | GAGGPHGETQ | 1/18 | 10 |
| T19/M0 | 2 | 2S5 | GRGGRNGRAQ | 1/18 | 10 |
| T19/M0 | 2 | 1S1 | RDSDRDNTEA | 2/18 | 10 |
| T19/M0 | 2 | 1S1 | NPGDSGYSPLH | 2/18 | 11 |
| T19/M12 | 2 | 1S2 | RTGGYGY | 1/9 | 7 |
| T19/M12 | 2 | 1S2 | SVPGNGY | 1/9 | 7 |
| T19/M12 | 2 | 1S1 | RDWDSEEA | 6/9 | 8 |
| T19/M12 | 2 | 1S1 | RDLKGLNTEA | 1/9 | 10 |
| T19/M20 | 2 | 1S1 | RGGIRA | 1/21 | 6 |
| T19/M20 | 2 | 1S1 | KQGNTEA | 1/21 | 7 |
| T19/M20 | 2 | 1S1 | RDWDSEEA | 11/21 | 8 |
| T19/M20 | 2 | 2S3 | RDLGQASW | 1/21 | 8 |
| T19/M20 | 2 | 1S2 | RKTGGAQN | 1/21 | 8 |
| T19/M20 | 2 | 2S6 | RDTDRANVL | 1/21 | 9 |
| T19/M20 | 2 | 1S5 | RDRQSNQPQ | 1/21 | 9 |
| T19/M20 | 2 | 1S1 | RDPPTASWYN | 1/21 | 10 |
| T19/M20 | 2 | 1S1 | TYRAISNQPQ | 1/21 | 10 |
| T19/M20 | 2 | 2S7 | RDGGFSTDTQ | 1/21 | 10 |
| T19/M20 | 2 | 1S1 | RETGGWGYTEA | 1/21 | 11 |
| T19/M0 | 7 | 2S3 | QDSGSEQ | 2/5 | 7 |
| T19/M0 | 7 | 1S1 | QAGGMRTEA | 3/5 | 9 |
| T19/M1 | 7 | 1S5 | LAAVSYEQ | 1/6 | 8 |
| T19/M1 | 7 | 2S5 | QGRSSYNEQ | 4/6 | 9 |
| T19/M1 | 7 | 1S1 | QAGGMRTEA | 1/6 | 9 |
| T19/M3 | 7 | 2S3 | QVQETQ | 1/8 | 6 |
| T19/M3 | 7 | 1S2 | HLTGAGGY | 1/8 | 8 |
| T19/M3 | 7 | 2S5 | QGRSSYNEQ | 6/8 | 9 |
| T19/M6 | 7 | 2S3 | QGTAINEQ | 1/9 | 8 |
| T19/M6 | 7 | 2S5 | QGRSSYNEQ | 6/9 | 9 |
| T19/M6 | 7 | 2S3 | QLYQFTYEQ | 1/9 | 9 |
| T19/M6 | 7 | 1S5 | QDGTGRRTPQ | 1/9 | 10 |
| T19/M12 | 7 | 2S5 | QGRSSYNEQ | 10/10 | 9 |
| T19/M20 | 7 | 2S5 | QGRSSYNEQ | 5/5 | 9 |
| T19/M0 | 8 | 1S1 | LWNTEA | 1/20 | 6 |
| T19/M0 | 8 | 1S1 | AGPGTEA | 1/20 | 7 |
| T19/M0 | 8 | 2S1 | LPGGSEQ | 1/20 | 7 |
| T19/M0 | 8 | 2S3 | LFWEYEQ | 1/20 | 7 |
| T19/M0 | 8 | 2S3 | LEEVYEQ | 1/20 | 7 |
| T19/M0 | 8 | 2S3 | SGPIYEQ | 1/20 | 7 |
| T19/M0 | 8 | 2S3 | LASGQETQ | 1/20 | 8 |
| T19/M0 | 8 | 2S1 | LTSAYNEQ | 1/20 | 8 |
| T19/M0 | 8 | 2S3 | FGGGADTQ | 1/20 | 8 |
| T19/M0 | 8 | 2S1 | GLLVLGDEQ | 5/20 | 9 |
| T19/M0 | 8 | 2S3 | LGQGTTYEQ | 1/20 | 9 |
| T19/M0 | 8 | 1S3 | RPRGSGNTI | 1/20 | 9 |
| T19/M0 | 8 | 2S3 | PSRDDSNTGEL | 1/20 | 11 |
| T19/M0 | 8 | 1S1 | LEDRDPRNTEA | 2/20 | 11 |
| T19/M0 | 8 | 1S2 | LLIGGQPFNYGY | 1/20 | 12 |
| T19/M20 | 8 | 2S3 | LFWEYEQ | 2/3 | 7 |
| T19/M20 | 8 | 2S1 | TQAGGDTQ | 1/13 | 8 |
| T19/M20 | 8 | 2S1 | LTTSAYEQ | 1/13 | 8 |
| T19/M20 | 8 | 2S1 | LGRGGNEQ | 1/13 | 8 |
| T19/M20 | 8 | 2S1 | GLLVLGDEQ | 4/13 | 9 |
| T19/M20 | 8 | 2S5 | LGQGTTYEQ | 1/13 | 9 |
| T19/M20 | 8 | 1S1 | LEDRDPRNTEA | 2/13 | 11 |
| T19/M20 | 8 | 1S2 | LLIGGQPFNYGY | 1/13 | 12 |
Sequences were deduced after DNA sequencing of the polymerase chain reaction products previously used for Immunoscope analysis (Figure 5). Redundant amino acid sequences, corresponding to identical DNA sequences and, thus, to clonal expansions, are in boldface.
aa indicates amino acid.