Flow cytometric analysis of fractional apoptotic responses in RNA-dependent protein kinase–overexpressing murine embryonic fibroblasts treated with interferon γ and double-stranded RNA
| Cells . | % Apoptotic cells . | ||
|---|---|---|---|
| Untreated . | dsRNA . | IFN-γ/dsRNA . | |
| Vector | |||
| FANCC+/+ | 0.48 (9940) | 0.15 (9950) | 1.15 (9820) |
| FANCC−/− | 0.28 (9906) | 1.84 (9967) | 10.01 (9645) |
| PKR | |||
| FANCC+/+ | 0.14 (9956) | 0.61 (9909) | 1.91 (9821) |
| FANCC−/− | 1.35 (9818) | 4.22 (9675) | 10.86 (9445) |
| PKRΔ6 | |||
| FANCC+/+ | 0.93 (9925) | 0.13 (9930) | 0.75 (9878) |
| FANCC−/− | 3.69 (9621) | 1.40 (9496) | 1.76 (9660) |
| Cells . | % Apoptotic cells . | ||
|---|---|---|---|
| Untreated . | dsRNA . | IFN-γ/dsRNA . | |
| Vector | |||
| FANCC+/+ | 0.48 (9940) | 0.15 (9950) | 1.15 (9820) |
| FANCC−/− | 0.28 (9906) | 1.84 (9967) | 10.01 (9645) |
| PKR | |||
| FANCC+/+ | 0.14 (9956) | 0.61 (9909) | 1.91 (9821) |
| FANCC−/− | 1.35 (9818) | 4.22 (9675) | 10.86 (9445) |
| PKRΔ6 | |||
| FANCC+/+ | 0.93 (9925) | 0.13 (9930) | 0.75 (9878) |
| FANCC−/− | 3.69 (9621) | 1.40 (9496) | 1.76 (9660) |
Murine embryonic fibroblasts carrying vector alone (pLXSH) or expressing (wtPKR) or PKRΔ6 were treated with murine recombinant IFN-γ (10 ng/mL) and/or 100 μg/mL of poly(I).poly(C) as described in Table 1. Numbers in parentheses are total events analyzed.
dsRNA indicates double-stranded RNA; IFN-γ, interferon γ; FANCC, Fanconi anemia group C; wtPKR, wild-type human RNA-dependent protein kinase.