Flow cytometric analysis of fractional apoptotic responses in murine embryonic fibroblasts treated with interferon γ and double-stranded RNA
| Cells . | % Apoptotic cells . | |||
|---|---|---|---|---|
| Untreated . | IFN-γ . | dsRNA . | IFN-γ/dsRNA . | |
| FANCC+/+ | 0.46 (3937) | 0.78 (4000) | 2.10 (4000) | 14.53 (4000) |
| FANCC−/− | 0.25 (4000) | 9.35 (4000) | 35.98 (4000) | 50.95 (4000) |
| Cells . | % Apoptotic cells . | |||
|---|---|---|---|---|
| Untreated . | IFN-γ . | dsRNA . | IFN-γ/dsRNA . | |
| FANCC+/+ | 0.46 (3937) | 0.78 (4000) | 2.10 (4000) | 14.53 (4000) |
| FANCC−/− | 0.25 (4000) | 9.35 (4000) | 35.98 (4000) | 50.95 (4000) |
Cells were incubated with murine recombinant IFN-γ (10 ng/mL) for 15 hours before transfection with or without 100 μg/mL of poly(I).poly(C) in the presence of LipofectAmine and harvested 24 hours after transfection for flow cytometric analysis as described in “Materials and methods.” The untreated cells were incubated with LipofectAmine only. Treatment of FANCC+/+ murine embryonic fibroblasts with camptothecin was used as a positive control (60 μM for 9 hours) and induced 11.8% positivity (2 916 events). Numbers in parentheses are total events analyzed.
IFN-γ indicates interferon γ; dsRNA indicates double-stranded RNA; FANCC, Fanconi anemia group C.