Table 5.
Recognition of substitution peptides by a cohort of T-cell clones from human leukocyte antigen A5-1500201 and cytomegalovirus-seropositive individuals
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TCCs were derived from individuals after in vitro stimulation using the native sequence of pp65495-503 as described in Figure 4. Limiting dilution cloning was conducted with a portion of the cells, and individual clones were evaluated using CRAs with the peptides shown above. The amount of peptide used to sensitize HLA-A
F5-150
0201–expressing EBVLCLs is expressed as either 5.0 or 0.5 nM. Specific cytotoxicity is shown as either greater than 30%, ▪; greater than 15% and less than 30%, ░; or less than 15%, ■.
For abbreviations, see Tables 1 and 4.