Table 4. Directed-substitution sequences... | American Society of Hematology

Table 4.

Directed-substitution sequences developed from peptides 44 and 46

	P1	P2	P3	P4	P5	P6	P7	P8	P9
Substitutions^4-150							V
	Y		L				T	S		T-cell clones
	N	L	V	P	M	V	A	T	V	3-3F4		VB57
Peptide name										−NH₂	−COOH	−NH₂	−COOH
193	Y	L	V	P	M	V	A	T	V	0.005^4-151	> 5	0.05	> 5
194	N	L	L	P	M	V	A	T	V	0.05	> 5	0.5	> 5
110	N	L	V	P	M	V	V	T	V	0.05	0.5	> 5	ND
111	N	L	V	P	M	V	T	T	V	0.05	0.05	> 5	5
112	N	L	V	P	M	V	A	S	V	0.5	5	0.5	0.5
116	N	L	L	P	M	V	V	T	V	0.5	> 5	> 5	ND
117	N	L	L	P	M	V	T	T	V	0.05	0.5	> 5	5
118	N	L	L	P	M	V	A	S	V	0.05	> 5	0.05	> 5
119	N	L	V	P	M	V	V	S	V	0.005	> 5	> 5	> 5
120	N	L	V	P	M	V	T	S	V	0.05	5	> 5	> 5
195	Y	L	L	P	M	V	A	T	V	0.005	> 5	0.5	> 5
113	Y	L	V	P	M	V	V	T	V	0.5	> 5	> 5	ND
114	Y	L	V	P	M	V	T	T	V	0.05	0.5	5	5
115	Y	L	V	P	M	V	A	S	V	0.005	> 5	0.05	5
126	N	L	L	P	M	V	V	S	V	0.005	5	0.5	> 5
127	N	L	L	P	M	V	T	S	V	0.05	5	> 5	> 5
124	Y	L	V	P	M	V	V	S	V	0.5	> 5	5	> 5
125	Y	L	V	P	M	V	T	S	V	0.05	> 5	> 5	> 5
121	Y	L	L	P	M	V	V	T	V	5	> 5	> 5	ND
122	Y	L	L	P	M	V	T	T	V	5	> 5	> 5	> 5
123	Y	L	L	P	M	V	A	S	V	0.05	> 5	0.5	> 5

	P1	P2	P3	P4	P5	P6	P7	P8	P9
Substitutions^4-150							V
	Y		L				T	S		T-cell clones
	N	L	V	P	M	V	A	T	V	3-3F4		VB57
Peptide name										−NH₂	−COOH	−NH₂	−COOH
193	Y	L	V	P	M	V	A	T	V	0.005^4-151	> 5	0.05	> 5
194	N	L	L	P	M	V	A	T	V	0.05	> 5	0.5	> 5
110	N	L	V	P	M	V	V	T	V	0.05	0.5	> 5	ND
111	N	L	V	P	M	V	T	T	V	0.05	0.05	> 5	5
112	N	L	V	P	M	V	A	S	V	0.5	5	0.5	0.5
116	N	L	L	P	M	V	V	T	V	0.5	> 5	> 5	ND
117	N	L	L	P	M	V	T	T	V	0.05	0.5	> 5	5
118	N	L	L	P	M	V	A	S	V	0.05	> 5	0.05	> 5
119	N	L	V	P	M	V	V	S	V	0.005	> 5	> 5	> 5
120	N	L	V	P	M	V	T	S	V	0.05	5	> 5	> 5
195	Y	L	L	P	M	V	A	T	V	0.005	> 5	0.5	> 5
113	Y	L	V	P	M	V	V	T	V	0.5	> 5	> 5	ND
114	Y	L	V	P	M	V	T	T	V	0.05	0.5	5	5
115	Y	L	V	P	M	V	A	S	V	0.005	> 5	0.05	5
126	N	L	L	P	M	V	V	S	V	0.005	5	0.5	> 5
127	N	L	L	P	M	V	T	S	V	0.05	5	> 5	> 5
124	Y	L	V	P	M	V	V	S	V	0.5	> 5	5	> 5
125	Y	L	V	P	M	V	T	S	V	0.05	> 5	> 5	> 5
121	Y	L	L	P	M	V	V	T	V	5	> 5	> 5	ND
122	Y	L	L	P	M	V	T	T	V	5	> 5	> 5	> 5
123	Y	L	L	P	M	V	A	S	V	0.05	> 5	0.5	> 5

ND indicates not determined; TCC, T-cell clone.

F4-150

The substitutions at each position are shown above the peptide sequences; this is given for positions that were varied in the library screen (Table 3) (ie, P1, P3, P7, and P8). Each peptide was made either with an amidated (NH₂) or free-acid (COOH) terminus.

F4-151

The concentration of peptide is that in which specific killing was between 15% and 30%. Peptides were tested using 10-fold dilutions between 500 and 0.00005 (3-3F4) or 0.005 (VB57) nM. The best peptides for each TCC and terminus are bolded.

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