T-cell and myeloid engraftment at TBI exposures of 750 cGy
| Experiment . | Donor T cells added . | GCV administration . | Percent donor . | |
|---|---|---|---|---|
| T cells . | Granulocytes . | |||
| 1 | None | No | 13-150, 1, 1, 53, 1 | 223-150, 4, 6, 98, 1 |
| 5 × 105 | No | 99, 54, 2, 99, 99, 100 | 95, 95, 65, 98, 98, 99 | |
| 5 × 105 | d 4-8 | 2, 2, 3, 79, 98, 7 | 12, 2, 12, 96, 96, 10 | |
| 2 | None | No | 2, 2, 1, 1 | 6, 92, 5, 19 |
| 5 × 105 | No | 100, 99, 3, 98, 99, 100 | 97, 99, 17, 98, 97, 97 | |
| 5 × 105 | d 8-12 | 97, 1, 99, 99, 80, 89 | 99, 6, 94, 96, 97, 92 | |
| Experiment . | Donor T cells added . | GCV administration . | Percent donor . | |
|---|---|---|---|---|
| T cells . | Granulocytes . | |||
| 1 | None | No | 13-150, 1, 1, 53, 1 | 223-150, 4, 6, 98, 1 |
| 5 × 105 | No | 99, 54, 2, 99, 99, 100 | 95, 95, 65, 98, 98, 99 | |
| 5 × 105 | d 4-8 | 2, 2, 3, 79, 98, 7 | 12, 2, 12, 96, 96, 10 | |
| 2 | None | No | 2, 2, 1, 1 | 6, 92, 5, 19 |
| 5 × 105 | No | 100, 99, 3, 98, 99, 100 | 97, 99, 17, 98, 97, 97 | |
| 5 × 105 | d 8-12 | 97, 1, 99, 99, 80, 89 | 99, 6, 94, 96, 97, 92 | |
Irradiated (750 cGy) AKR mice were transplanted with either TCD B6.PL BM alone (107 cells) or together with 5 × 105 B6 TK+ T cells. A cohort of mice in each experiment transplanted with TK+ T cells was treated with GCV (50 mg/kg per day) for 5 days after transplantation on the specified scheduled noted above. Animals were bled on day 28 in experiment 1 and day 29 in experiment 2.
One mouse in group 1 of experiment 1 was bled on day 18 immediately prior to demise.